响应面法优化超声波辅助提取湖北海棠叶中总黄酮工艺.pdfVIP

※王艺技术 20日~ Vo1.34, No.02 143 自由融事 响应面法优化超声波辅助提取湖北海棠叶中 总黄酣王艺 乔孟，屈晓请，丁之恩* (安徽农业大学茶与食品科技学院，安徽合肥 230036) 摘 要z 采用超声波辅助提取湖北海棠叶中总黄圃，并利用响应面法对提取工艺参数进行优化。在料液比、乙醇体 积分数、超声时间、超声功率等单因素试验基础上，进行四因素三水平的Box-Behnken设计，利用响应面法分析4个 因素对湖北海棠叶中总黄酣得率的影响。超声波法提取湖北海棠叶中总黄酣的最佳工艺条件为液料比50: 1(mLlg)、 乙醇体积分数67.35%、超声时间3.2h、超声功率162.4W，总黄酣得率预测值为12.78%，验证值为12.76%，与理论 值的相对误差为0.02%，表明该优化方法合理可行。 关键词:湖北海棠叶:总黄酣:超声波辅助提取:响应面优化 Optimization of Ultrasonic-Assisted Extraction Process for Total Flavonoids from Ma/us hupehensis Leaves by Response Surface Methodology QIAOMe吨， QU Xiao-qi吨， DING Zhi-en* (College of Tea and F，∞d Science and Technology, Anhui Agric旧tural Univers坷， Hefei 23∞36， China) Abstrad: Response surface methodology (RS岛的 was applied to optimize conditions for the ultrasonic-assisted extraction of tota1 flavonoids from Malus hupehensis leaves. One-factor-at-a-time method was used to explore the effects of solvent- to-solid ratio , e白血01 concentration , ultrasonic treatment time and ultrasonic power on extraction efficiency. Based on 出is， these process conditions were optimized using a four-variable , three-level Box-Behnken experimenta1 design couple with response surface analysis to be 67.35% ethanol as 由e extraction solvent at a solvent-to-solid ratio of 50:1 (mUg) and ultrasonic treatment for 3.2 h at 162.4 W. Under the optirnized conditions, the predicted and actual yields of tota1 flavonoids were 12.78% and 12.769毛 with a relative error of 0.02%. 咀lerefore，由e optimized extraction procedure is reliable. Key words: Malus hupehensis; tota1 flavonoids; ultrasonic-assisted extraction; response surface methodology 中图分类号: R284.2 文献标志