苯肾上腺素延长糖尿病大鼠心脏QT间期的机理研究.pdfVIP

中药药理与临床2007；23(6) l7 一 six male SD rats were randomly divided into normal group，model group，vehicle group，paracetamol group and madecassoside l Omg／kg， 20mg／kg，40mg／kg．Fever model of rat was induced by ip LPS(100 g／kg)．The changes of deep body temperature(AT)Was tested every 30 min after ip LPS，and lasted for 6 h．Madecassoside(10，2O and 4O mg／kg)Wills ig once every day and last 3 days before ip LPS．The rats of normal and model groups were ig normal saline，vehicle group were ig 0．5％ carboxymethyl cellulose instead of madecassoside．Paraceta— mol group rats werc ip paracetamol(50 mg／kg)only once，30 min late1‘ip LPS．the activity of MPO in live1。Wills detected by myeloperoxidase (MPO)kit；the content of PGE2 in brain and IL一6 in plasma was detected by ELISA．Results：The fever was appearance three febrile phase，the ma~mum amplitude of temperature achieved 2．01 oC．Compared with model group，madecassoside(1 0，2O and 40mg／kg)have the effection of hypothermia obviously，and madecassoside 40 mg／kg inhibite the febrile is similar to the paracetamol group．compared with model group，madecassoside(10，20 and 40mg．kg一1)reduce the activity of MPO in liver and the content of PGE2 in brain，IL一6 in plasma obviously．Condusion：Madecassoside have the effection of hypothermia，and this effects maybe is related to inhibite the express of MPO ． PGE2 and IL一6． Key words madecassoside；lipopolysaccharides；fever；IL一6；MPO；PGE2 苯肾上腺素延长糖尿病大鼠心脏QT间期的机理研究 陈 龙 王玲玲 ，徐 斌 ，余 黎 ，韩 冰 ，王 芳 ，卞慧敏 ，朱 荃 ( 中医药研究院； 针药结合实验室；南京中医药大学，南京 210029) 摘 要 目的：分析苯肾上腺素显著延长糖尿病大鼠心脏QT间期的钾电流机理。方法：采用膜片钳全细胞式的记录方法， 观察苯肾上腺素对正常及糖尿病大鼠左心室肌细胞的瞬时外向钾电流(Ito)及稳定状态的钾电流(Iss)的差异作用。结果：与对正 常动物的作用相比，苯肾上腺素降低正常的和糖尿病大鼠的瞬时外向钾电流(Ito)，和稳定状态的钾电流(Iss)。但与正常大鼠相 比，降低糖尿病大鼠稳定状态的钾电流(Iss)更明显。结论：与正常动物相比，苯肾上腺素明显延长糖尿病大鼠离体心脏QT问期及 动作电位复极90％水平时程(APD ，)是由于其更显著降低左心室肌细胞稳定状态的钾电流(Iss) 关键词 苯肾上腺素；膜片钳；钾电流 苯肾上腺素(Phenyl