药物靶标的发现与验证技术研究进展论文.docVIP

　　药物靶标的发现与验证技术研究进展论文 【摘要】 药物靶标的发现和验证是新药研发的第一步，也是药物筛选及药物定向合成成败的关键因素之一。随着基因组学、蛋白组学、生物信息学的快速发展，以及RNA干扰、噬菌体展示等新兴技术的出现，药物靶标的发现和验证技术日新月异。该文综述了在基因水平、转录水平、蛋白水平药物靶标发现和验证的各种技术的研究进展及其成功案例。 【关键词】 药物靶标;靶标发现;靶标验证 Abstract：Drug target discovery and verification is not only the first step of investigation of neost important factors in drug screening and directional synthesis. , Melendez AJ. DNA microarray technology for target identification and validation J. Clin Exp Pharm Physiol, 2006, 33 (5):371. 3郑越, 程肖蕊, 周文霞, 等. DNA微阵列技术在阿尔茨海默病及其防治药物研究中的应用J. 生物技术通讯, 2007, 18 (2): 320. 4Grunblatt E, Mandel S, Maor G, et al. Gene expression analysis in N-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine mice model of Parkinson′s disease using cDNA microarray: effect of R-apomor-Phine J. J Neurochem, 2001, 78 (1): 1. 5Stam RL, Meijerink JP, et al. Differential mRNA expression of Ara-C-metabolizing enzymes explains Ara-C sensitivity in MLL gene-rearranged infant acute lymphoblastic Leukemia J. Blood, 2003, 101(4): 1270. 6Thal MA, Carvalho TL, He T, et al. Ebf1-mediated doice J. Biochem Biophys Res mun, 2009, 378 (4): 851. 8Cao RY, St. Amand T, Gr?bner R, et al. Geic and pharmacological inhibition of the 5-lipoxygenase/leukotriene pathent in ApoE deficient mice J. Atherosclerosis, 2008, 203 (5): 395. 9Sun Z, Cade R, Zhang Z, et al. Angiotensinogen gene knockout delays and attenuates cold-induced hypertension J. Hypertension, 2003, 41(2):322. 10 Chen ol Cell Biol, 2009, 29 (11): 3151. 11Rao CV, Lei ZM. Consequences of targeted inactivation of LH receptors J. Mol Cell Endocrinol, 2002, 187 (1): 57. 12 Jarvis MF, Burgard EC, McGaraughty S, et al. A-317491, a novel, potent and selective non-nucleotide antagonist of P2X3 and P2X2/3 receptors reduces chronic inflammatory and neuropathic pain in rat J. Proc Natl Acad Sci USA, 2002, 99 (26):17179. 13Okabe H, Furukaent and differentiation enhancing factor-like 1 (DDEFL1) as a drug target for hepatocellular carcinomas J. Int J Oncol, 2004, 24 (1): 4