PKCa 中国医科大学(英文版).pdfVIP

J Mol Neurosci DOI 10.1007/s12031-011-9584-5 Recombinant Human Angiopoietin-1 Ameliorates the Expressions of ZO-1, Occludin, VE-cadherin, and PKCα Signaling after Focal Cerebral Ischemia/Reperfusion in Rats Hang Yu Ping Wang Ping An Xue Yixue Received: 10 April 2011 /Accepted: 13 June 2011 # Springer Science+Business Media, LLC 2011 Abstract This study was performed to determine whether groups. The BBB permeability was assessed by Evans blue recombinant human angiopoietin-1 (Ang-1) decreases the extravasation. The messenger RNA and protein expressions of permeability of the blood–brain barrier (BBB) in focal ZO-1, occludin, and VE-cadherin were determined by reverse cerebral ischemia and reperfusion rats, whether Ang-1 transcription-polymerase chain reaction, western blot, and opens the BBB by affecting tight junction associated immunohistochemistry assays. The BBB permeability was proteins zonula occluden-1 (ZO-1), occludin and adherens significantly decreased after Ang-1 injection. The expressions junction protein vascular endothelial (VE)-cadherin, and of ZO-1, occludin, and VE-cadherin were increased after Ang- whether the protein kinase C (PKC)α/myosin light chain 1 injection. These were in accordance with the results of (MLC) signaling pathway involves in it. The rats were immunohistochemistry assays. PKCα and phosphorylated divided into eight groups randomly: (1) sham-operated MLC (p-MLC) expressions were decreased after Ang-1 group, (2) ischemia group, (3–5) ischemia–reperfusion injection. This study demonstrated that Ang-1 may decrease (middle cerebral artery occlusion and reperfusion (MCAO/R) the permeability of BBB in MCAO/R rat by upregulation of 12 h