LVLX-SDS体系的荧光特性及左氧氟沙星的测定.pdfVIP

• 689 • 中国统生素杂志 2008 年))月第 33 卷第 11 期 31:.编4i} dω1-8689(2008)1 1心689-03 LVLX-SDS 体系的荧光特性及左氧氟沙星的测定 蔡花真刘彦牵1) *会平谢红涛 〈河南质量工程职业学院， 平顶山 467∞1) 摘要z 目的 研究不同表西活性llIJ 对左氯氟沙皇Oevofloxacin ,LVLX)荧光强度的影响.建立表面活性剂增敏.荧光光度法测 定左氯氟沙爱含量的新方法. 方法 比较不闵表面活性剂对左氧氟沙皇荧光符性的影响，发现在pH6.0 的缓冲溶液中十二统!I;硫 峻销(SDS)对左氯氟沙皇荧光强度有显著的精敏作用.建立SDSj曹敏.荧光光度法快速测定左氧氟沙庭的新方法.结果 将该方法 用于左氧氟沙皇片剂和注射液的事定，结果与国标检测方法离效液相色谱(HPLC)法一致.样品平均回收率为 1ω.4Y RSD 为 0.64%.撞出限为 18.9μg/L. 篇论该方法简便、快捷、准确. 关键词z 左氧氟沙皇， 十二饶事E硫磁锅， 荧光测定法 中国分类号: R978. 1 文献标识码;A The fluorescence characteristic of LVLX-SDS and the determination of levofloxacin Cai Hua-zhen , Liu Yan-zhao , Xi Hui-ping and Xie Hong-tao (Henan Quality Polyttthnic. Pingdingshan 46700 1) ABSTRACT Objectlve Effect of diffcrent surfactants on the fluorimetric intensity of !evofloxacin (!.VLX) were studied. We established a new method for quantitative determination of levofloxacin. Methods Effect of different surfactants on the fluorimetric intensity of levofloxacin have been compared. We discovered sodium dodecyl sulfate could enhance the fluorimetric intensity of levofloxacin . Based on this reaction , lev- ofloxacin was determined by fluorescence spectrophotometry. Results The reaction conditions were investi- gated in detai l. ln LVLX-SOS system , the linear range was 54 ， 500μg/L and regression equation was F= 1.34339+O. 093 1lC (严g斤_) (r= 0.9994). The average recovery of levofloxacin was 100.4% with RSO of 0.64%. Conclusion This method W8S simple , 8ccurate , rapid and could be used to determine ciprofloxacin in tablet and injection. ,