MAE-GCMS法测定食品中B(α)P.pdfVIP

噩噩罩噩噩 MAE-GCIMS 法测定食品中B(α)P 蓝长波 { 柳州食品药品给钱所. ，.. 两柳州 545∞6) 摘要: 建立了测定食品中苯并花(B(α)P)含量的 MAE-GC/MS 法。 样品经橄波辅助萃取技术前 处理，DB-5MS 弹性石英毛细管柱(30 mxO.25 mmxO.25μm)分惑，以 B(α)P mlz=252 为选择离 子，串连四极杆选择离子检测模式检测，再以 9-苯基葱为内标，采用内标法定量。 结果显示­ B(α}P 的检出 fll 为 0.01 ~g/kg，标准曲线线性范因为 0-10 ~g/mL，相关系数为 1.0∞，方法回收 率达 97%-103%;精密度良好:日内 RSDO.5%，日 间 RSOI %; 准确皮良好:以阴性样品为基底 物质，在 0.1 mL 甲醇苯并花成分分析国家标准物质(GBW08702) 的加标水乎上，测定位在其标 准位范围内。 表明:该法前处理简单，回收率高，捻tl:限、 线性范阁、准确度、 柑密皮结采均满足妥 求，可用于测定食品中的 B(α)P 含量。 关键词: 食品;微波辅助革取技术;气相色谱/质谱 中图分类号:R 155.5 文献标志码:A 文章编号: 1 673一1689 (2014) 12一1332-06 MAE-GC瓜1S Determination Foods B (α) P Content LAN Changbo (food and Orug Control of Guangxi Liυzhou •Liuzhou 545006 , Chinll) Abstract: The aim of this study is to develop MAE-GC/MS method to detennine the Benzo (α) pyrene (B(a}P) contenl ror this ,the sampJe was pre-treatment by MAE(microwave-assisted extraction technology }and th en separate by ,OB-5MS fused süica capillary column (30 m地25 mmxO.25μm) with B(α)P mlz=252 asthe selectecl ion ,the detection is the series four quadrupole selected ion monítoring (SIM ) mooe with 9-phenyl anlhracene as intemal standard. A 臼ries of results were achieved: B(α)P detection limil was 0.01 ~g/kg and the standard curve was línear at the range of 0- 10 ~g1mL. The correlation coefficient was 1.∞0 ， with the recoveries of 97%-103% and good precision: Days RSD/% 0. 5% ,day RSD/% 1 %. The good accuracy , the negative sample as lhe base material , containing 0.1 mL methanoJ and benzo (α) pyrene national standard component analysis (GBW08702) spiked levels ,the measured values with in the stand