UPLC法测定宣木瓜中齐墩果酸、熊果酸.pdfVIP

南京中医药大学学报 2013 年 5 月第 29 卷第 3 期 JaRNALCFN也飞JIN:;(川瓦恒RSI7Y CF TCM Vi. æN.3 Mly 3)13 UPLC 法测定宣木瓜中齐墩果酸、熊果酸 沈盼盼，谢晓梅祷，张圣龙，朱晨晨 (安徽中医学院现代中药安徽省重点实验室，安徽合肥 230031) 摘要:目的 对高效液相色谱法(HPLC)进行转换并优化，建立超高效液相色谱法(UPLC) 测定宣木瓜中齐墩采酸(OA) 和熊 采酸(UA)含量，并与 HPLC 测定法相比较。方法 UPLC 采用 ACQUITY UPLC™ BEH C 色谱柱(1 00 mmX2.1 mm , 1. 7 18 μm) ，流动相:甲醇-水;水乙酸-三乙接(254 : 46 : O. 1 : O. 05) ;流这 :0.3 mL/min，检测波长: 210 nr口，柱温 :25 C ，进样体积: 1 μL;HPLC 色谱柱为 Shim-pack CLC-ODS(M) (250 mmX4. 6 mm， 5μm) ，流动相:甲醇-水-冰乙酸-三乙膝(265 : 35 : O. 10 : 0.05); 流速:0.6 mL/min; 柱温及检测波长同 UPLC;进样体积:20μL。结果 2 种方法测定不同采收期宣木瓜中 OA 和 UA 含量，结果基本一致;UPLC 法分析时间短，溶剂消耗少，检测灵敏度高。结论 UPLC 法可替代 HPLC 法测定宣木瓜药材中 OA 和 UA 含量。 关键词:超高效液相色谱法;高效液相色谱法;宣木瓜;齐墩果酸;熊果酸 中图号:R284.1 文献标志码:A 文章编号:1672 -0482(2013)03 -0271-03 Detecting Oleanolic and Ursolic Acid with UPLC in Chaenomelis Fructus from Xuancheng SHEN Pan-pan ,XIE Xiao-mei , ZHANG Sheng-long, ZHU Chen-chen (Anhui College of Traditional Chinese Medici肘， Anhui Key Laboratory of Modern Chinese Materia Medica , Hefei , 230031 , China) ABSTRACT:OBJECTIVE To transform and develop high performance liquid chromatograph( HPLC) , to establish ultra per- formance liquid chromatography (UPLC) for determinating the contents of oleanolicacid (OA) and ursolicacid (UA) in Chaenomelis Fructus and to compare UPLC with HPLC. METHODS In the UPLC , ACQUITY UPLC TM BEHC18 column (1 00mmX2.1mm，1. 7μm) was adopted , and the column temperature was 25C. The mobile phase consisted of methanol-wa ter-glacial acetic acid-triethylamine (254 : 46 : 0.1 : 0.05) with a flow rate of O. 3mL/min. The detection wave length was 210nm , and injection volume was 1μL in UPLC system. A Shim-pack CLC-ODS( M) column (250mm X 4. 6mm , 5μm) was u- tilized in HPLC system , and a mixture of methanol-water-glacial acetic a