UPLC法进行柴胡疏肝散体内外成分的同步定性测定.pdfVIP

-E 百· J Med Res ,Jul20 1O,Vol. 39 No.7 UPLC 法进行柴胡疏肝散体内外成分的 同步定性测定 胡思航黄熙粱清华1-锋邱新建任f-范荣唐涛张春虎 摘 要 目的 进行柴胡疏肝散汤剂中多成分及其激胃该方厉大鼠小肠中吸收成分的同步定性测定。方法 建立 UPLC 呻 PDA 测定的新方法，阿步定性测定:然胡疏肝散汤剂中的 11 种成分;灌商(35g1kg) 该方 30 min 后健康大鼠小肠中的吸收成分; 色谱条件为 ACQUITY UPLC BEH C 校(2.1mm x50mm ,1. 7μm) ，流动相为乙脯叩0.5% 醋酸水，流量:0.5mνmin ，温度 :40C ，检 18 结果 测波长:190 何 480nm 41mín 内，该方中附魏酸、将药背、袖皮管、橙皮管、水合橙皮内醋、新橙皮背、界甘率索、台;草酸 在 o 镀、苟药内醋背、甘掌管和甘革次酸 12 种成分及灌留该方30mín 后健康大鼠小肠中的上述前 8 种成分获得良好分离，各成分的 3 LOD 在 0.166 X 10- -1. 44μν时，信噪比(5/N);!!: 3 结论 该方法快速、灵敏:上述的结果为该方进行质量控制及其药动学研 0 究奠定了方法学蔡础。 UPLC PDA SD 大鼠小肠定性测定 关键词柴胡疏肝散 Slmultaneously Qualitatlve Determination or Multlple Compounds in Chaihu Shugan san( CSS) and in Rat Int制tine by UPLC 翻 PDA. Hu Síhα略， Huαng Xí , Liang Qínghua , Qin Feng , Qiu Xinji棚， Ren Ping , Fan Ro咐， Tang Tao , Zhang Chunhu. Lαboratory 01 Ethnopharma- cology , Xiangya Hospital , Central South University , Hunan 410008 , China Abstract Objective To simultaneously qualitatíve detenninate the multí - component ín Chaíhushugansan ( C55) and ín íntestine in rat following oral C55. Methods A novel UPLC - PDA method was developed to qualitate símultaneously 11 compositíons in C55 and absorbed components in int嗣tine 30 min after oral C55 in healthy 5D rats (35 glkg). The chromatographic conditions: analytical column was a Waters BEH (R) C18 Column (2.1mm x 50mm i. d. , particle size 1. 7μm). The mobile phase contained A acetonitrile and B - 0.5% acetic acid. The f1 0w rale was O. 5m l/min