第01章蛋白质化学 - 华南师范大学生命科学学院.ppt

绪 论 Some Rules or Suggestions Turn off your pager or cell phone when in class Don’t take notes too seriously, Just focus on listening and understanding Either you come here on time or you won’t Don’t ask me for mercy when you get poor grades in your exam Introduction to Biochemistry What is Biochemistry? Bio + Chemistry= Biochemistry The Goals of Biochemistry Describe structure, organization, function of cells in molecular terms (1) Structural Chemistry(2) Metabolism(3) Molecular Genetics A Tip on how to study Biochemistry well Just do as a proverb says: I hear, and I forget, I see, and I remember, I do, and I understand. Amino acid structure Different side-chain (R group) Different chemical and physical properties Amino Acid Classification Aliphatic Aromatic Sulfur containing Polar/uncharged basic/acidic Abbreviations for 20 amino acids alanine - ala - A arginine - arg - R asparagine - asn - N aspartic acid - asp - D cysteine - cys - C glutamine - gln - Q glutamic acid - glu - E glycine - gly - G histidine - his - H isoleucine - ile - I leucine - leu - L lysine - lys - K methionine - met - M phenylalanine - phe - F proline - pro - P serine - ser - S threonine - thr - T tryptophan - trp - W tyrosine - tyr - Y valine - val - V asparagine/aspartic acid - asx - B glutamine/glutamic acid - glx - Z UV-absorbing Properties of Amino Acids Only three amino acids, Phe, Tyr, and Trp, absorb light in the near UV range (230 nm-300 nm). These amino acids dominate the UV absorption spectra of proteins. The wavelength maxima for tyrosine and tryptophan are around 280 nm. In contrast, nucleic acids (we will discuss later) have absorption maximum of 260 nm. Thus a simple UV scan can allow one to distinguish between protein and nucleic acids. Titration Curve for Alanine Titration Curve for Glutamic Acid Titration Curve for Lysine Reaction with Ninhydrin Used to vis