劳氏紫瑞利共振散射荧光法测定肝素钠.pdfVIP

何地大学学报(俞然科学版) 2014 2014 年 第 34 卷第1 期 Journal of Hebei University (Natural Science Edition) Vo 1. 34 No. 1 DOI: 10. 3969/j. issn. 1000 -1565. 2014. 0 1. 010 劳氏紫瑞利共振散射荧光法测定肝素纳 李志云，刘保生 (河北大学化学与环境科学学院，河北保定 071002) 摘 要:近中性的 BR(Britton-Robinson， H P0 -HAc-H B0 ) 缓冲溶浪中，肝素纳与劳氏紫形成离子 4 3 3 3 缔合物，在 564 nm 处生成新的瑞利共振散射(RRS)峰，随着肝素铀浓度的增加 RRS 峰显著增强，据此建立 了劳氏紫瑞利共振散射荧光测定肝素铀的新方法.该方法在 O. 025~0. 500 mg/L 内 RRS 强度与肝素纳浓 度主良好的线性关系，线性回归方程为 t:. I =0.125十16. 88p(mg/U. 用于肝素纳注射液含量测定，平行6 次 测定，相对标准偏差为1. 67%~3. 01% ，方法回收率为 104. 0%~106. 4% ，结果满意. 关键词:肝素销;劳氏紫;瑞利共振散射p 测定 中图分类号:0657.32 文献标志码:A 文章编号: 1000 - 1565 (2014)01 - 0053 一 04 Determination of heparin sodium with thionine by fluorescence and resonance rayleigh scattering LI Zhìyun , LlU Baosheng (College of Chemistry and Environmental Sciences , Hebei University ,Baoding 071002 , China) Abstract: 1n the near-neutral BR buffer solution , a new RRS spectrum was found at 564 nm about heparin sodium and thionine after the formation of ion-association complex. ancl in a certain range , RRS in tensity significantly increased with the addition of heparin sodium. Based on this , a novel method for the determination of heparin sodium was developed. The results showecl that the RRS intensity of the system was proportional to the concentration of heparin sodium in the range of O. 025-0. 500 mg/L and the linear regression equation was t:.I = O. 125 十16.88p (mg/L). Meanwhile , the relative standard