生物钟PER3 基因多态性和乳腺癌易感性相关性研究.docVIP

生物钟PER3基因多态性与乳腺癌易感性的相关性研究 The Association between the Circadian Gene PER3 Polymorphism and the Susceptibility of Breast Cancer 曹明丽1 戴弘季2 宋丰举2 郑红2 陈可欣2* Mingli C 1, Hongji D 2, Fengju S 2, Hong Z 2, Kexin C 2* 作者单位：1.天津市河西区疾病预防控制中心 慢病科 天津市300211 2天津医科大学附属肿瘤医院肿瘤研究所 流行病室 天津市300060 国家自然基金项目（编号 The Association between the Circadian Gene PER3 Polymorphism and the Susceptibility of Breast Cancer 【Abstract】 Objective To explore the relationship between the polymorphism of the PER3 gene and the susceptibility of the breast cancer in Chinese population, Methods A case-control study was conducted, including 836 cases of breast cancer and 946 controls, PER3 gene polymorphisms were determined with polymerase chain-restriction (PCR). Results Women with a marry age more than 25 years old, a higher education level , abortion, breast benign disease, family cancer history, smoking and rich fatty intake could significantly enhance the risk of breast cancer. Late menarche, more birth and vegetable intake could significantly decrease the risk of breast cancer. And we found that the variant PER3 genotype (homozygous 5-repeat alleles) can enhance the risk of breast cancer, compared with the PER3 genotype (homozygous + heterozygous 4-repeat alleles) (OR=3.503, 95%CI: 1.069~11.480, OR=3.337, 95%CI: 1.025~10.864) adjusted by related factors. Conclusion The PER3 genotype (homozygous 5-repeat alleles) could significantly enhance the risk of breast cancer. Key words: breast cancer, PER3, Circadian gene, Genetic susceptibility, biorhythms 生物钟PER3基因多态性与乳腺癌易感性的相关性研究 【摘要】 目的 探讨乳腺癌发生的主要环境危险因素和参与生物节律调控的生物钟PER3基因多态性与乳腺癌易感性的关系。方法 采用病例-对照研究设计的分子流行病学研究，836例乳腺癌患者和946例对照，采用 PCR 技术对 PER3 基因多态性进行检测，结果 结婚年龄超过25岁、文化程度高、人工流产史、乳腺良性疾病史、癌症家族史、吸烟、肉类摄入量多能显著增加乳腺癌发生的危险性，均有统计学意义；初潮年龄晚（大于15岁）、活产数多（大于1）、蔬菜摄入量多能显著降低乳腺癌发生的危险性，有统计学意义。经相关因素调整后，PER3 基因型与乳腺癌的多因素Logistic回归分析发现，携带PER3 +/+ 基因型的研究对象发生乳腺癌的危险是携带PER3 -/- 基因型对象的3.503倍（OR=3.503，95%CI：1.069~11.480），是携带PER3 +/- 或PER3 -/- 基因型对象的3.337倍（OR=3.337，95%CI：1.02