血水草生物碱对钉螺肝脏超微结构的影响.pdfVIP

实用预防医学 2007年12月 第 14卷 第6期 Practical Preventive Medicine，Dec．2007，v0l 14，No．6 1675 文章编号：1006—3110(2007)06—1675—03 【论 著 】 血水草生物碱对钉螺肝脏超微结构的影响 彭 飞，黄琼瑶，刘年猛，孙 慧，刘 铭 摘要： 目的 钉螺经血水草生物碱水溶液浸泡后，观察肝脏超微结构的变化，探讨血水草生物碱杀灭钉螺的作用 机理。 方法 用血水草生物碱浸泡钉螺48 h，解剖钉螺，取肝脏用常规方法制作电镜切片，用日立 H一600型透射电镜 观察组织结构变化。 结果 血水草生物碱浸泡后，钉螺肝细胞肿胀、变性，内质网扩张呈空泡状。线粒体嵴肿胀 模糊 不清，部分嵴断裂或丢失，呈空泡变；毛细胆管扩张；部分细胞核坏死出现溶解或萎缩；绒毛减少、不规则或消失。 结论 肝细胞严重损伤是血水草生物碱致钉螺死亡的重要原因之一。 关键词： 血水草生物碱；钉螺；肝脏；超微结构 中图分类号：R383、2 4 文献标识码：A Eff_ects of Eomecon Chionantha Hance Alkaloi(Is Oil Ultrastructure of Liver Cell of Oncomelania hupensis PENG 西． HUANG Qiong—yao，LIU Nian—mcag，et a1．(Medical College ofHunan Normal University，Changsha 410013，Hunan， China) Abstract： 0bjective To explore the mechanism of eomecon chionantha hance alkaloids(ECA)against Oncomelania hu— pensis by means of observing the effects of ECA on the liver cell ultrastructure of O．hupensis． Methods 0ncomelania hu- pensis was dissected after immersing in ECA solution for 48 hrs，liver tissues were dissected to prepare conventionaI electron microscopy sloecimens and the changes of liver cell ultrastructure were examined by Hitach H一600 Transmission Electron Mi- croscope． Results After immersing in ECA solution；the liver cell was swollen and degenerated．The rough endeplasma reticulum(RER)expanded，and some of the mitochondrial ridges were swollen，destroyed，vesiculated and disappeared．The bile canaliculus lumen was enlarged．Some of the cell nuclei were atrophied，coagulated or dissolved，microvilli between the cells near the gland channel diminished and became irregular and vanished、 Conclusions The liver cell ultrastructure of O． hupensis is severely damaged after immersing in ECA solution．The results suggest that damaged liver cell