低能离子束促进小麦copia-反转录转座子的转录与转座.pdfVIP

JournalofPlantPhysiologyandMolecularBiology 2007，33(6)：507-516 Low-Energy Ion Beam um aestivum L) YA Hui—Yuan ，GU Yun—Hong ，JIAO Zhen ，WANG Wei—Dong ， QIY Guang—Yong ，HUO Yu．Ping HenⅡn Key Lab oflon。beam Bi。PnginPPrin g，Zhengzh。“University,Zhengzh。“450052,China； CollegP ofLife SciPncP LuoyangⅣ0，，，lnz f f班 Luoyang 471022，China Abstract：LTR—retrotransposons are genetic elements et a1．1999；Meyers et a1．20011．TEs f l into two classes： having the direct long terminal repeats(LTRs)．It can DNA transposons and retrotransposons ． The latter can move via an RNA intermediate within genomes and is be transposed through reverse transcription of an inter— an important fraction of eukaryote genomes．Low—energy mediate RNA(Grandbastien 1992；Kumar and Bennetzen N ion beam promoted the transcription of the copia． 1999)．Retrotransposons are ubiquitous in plant genomes retransposons in those wheat(cv．‘Zhoumai 16’)，which (Kubis et a1．1998)．They are classified into two major were radiated and allowed to grow for 24 h and 48 h families，the U1R(1ong terminal repeat)and the non—U1R fromthe~anting．Relative expression ratioofthe cop／a． retrotransposons，basing on the presence or absence of retransposons was elevated in different degrees(with a L11Rs at the end of transposons(Boeke and Corces 1989)． max 40 fold)in wheat plants treated with different doses L11Rretrotransposons arefurther subdividedintotheTv1一 of N+beam。comparing to that in the controls ． The mol— copia and the Xy3一gypsy groups(Casacuberta and eculemarkersoftheIRAPandREMAPtotheDNAiso—