化维纤胶囊中腺苷含量的测定.pdfVIP

中国卫生检验杂志2007年12月第l7卷第l2期 Chinese journal of Health Laboratory Technology，Dec 2007；Vol 17 No 12 2233 【化学测定方法】 化维纤胶囊中腺苷含量的测定 谭莹，韩见龙，张晶，冯靓，蔡增轩，任一平 (浙江省疾病预防控制中心，杭州 310009) [摘要] 目的：建立化维纤胶囊中腺苷的HPLC测定方法。方法：用不同提取试剂提取后，以HPLC法测定腺苷含量及 加标回收率，选择最佳提取液，并作方法学论证。结果：以纯水作为提取试剂，腺苷在0 84～66．96 ml的线性范围内， 回归方程Y=68700X一18500(r=0．9999)。最低检出浓度为0．02 mg／100 g；当样品添加浓度为8．37—41．85 mg／100 g 时，方法回收率为95．9％～102．1％。结论：以纯水提取化维纤胶囊中的腺苷，操作简便、定量准确可靠。 [关键词] 腺苷；化维纤胶囊；HPLC；提取液 [中图分类号] 0657．7 2 [文献标识码] A [文章编号] 1004—8685(2007)12—2233一O3 Determination of adenosine in Huaweixian capsule Tan Ying，Han Jian—long，Zhang Jing，Feng Liang，Cai Zeng一姗 n，Ren Yi-ping (Zhejiang Center for Disease Control and Prevention，Hangzhou 3 10009，China) [Abstract] Objective：To establish an HPLC method for quantitation of adenosine in Huaweixian capsule．Methods：Select the best extract solvent from severe candidates according to determined added standard recoveries and content of adenosine．Then do the HPLC methodology demonstration using selected best extract solvent．Results：The method using purified water as extract sol— vent exhibited a linear relation within 0．84—66．96 ml adenosine content．The regression equation was Y=68700X一1 8500(r =0．9999)and the minimum detectable limit of this method was 0．02 mg／100 g．Furthermore，the recoveries of adenosine were 95．9％ 一102 1％ while the contents of added standard were 8．37～41．85 mg／100 g．Conclusion：This method using purified water as extract solvent is simple，accurate and reliable for determination of Adenosine in Huaweixian capsule． [Key words] Adenosine；Huaweixian capsule；HPLC；Extract solvent 化维纤胶囊是近年来新兴的一种保健品，有调节免疫力、 1．2 液相色谱务件 抑制致纤维化因子分泌、逆转组织纤维化等功效，并起到保护 色谱柱：Phenomenex—Cl 8 ODS柱(4．6