去细胞组织工程心脏瓣膜构建的实验研究.pdfVIP

2007年第21卷第6期—Med J of Communieations,2007,Vo1．21．No．6 ·63卜 [文章编号】1006—2440(2007)06—0631—03 去细胞组织工程心脏瓣膜构建的实验研究 蒋红亮 武秀华 黄利荣 (盐城市第一人民医院心胸外科，江苏224001； 上海市第六人民医院肺内科) 摘要 目的：探讨人脐静脉内皮细胞(HUVECs)种植在去细胞瓣叶上构建组织工程心脏瓣膜的可行性。方法： 测定瓣叶去细胞前、后的生物力学性能；观察去细胞瓣叶上内皮细胞生长情况。结果：瓣叶去细胞可获得完整无细 胞的纤维支架，瓣叶去细胞前、后的生物力学性能无差异；内皮细胞在去细胞瓣叶表面生长，形成一层基本连续的 细胞层。结论：去细胞瓣叶组织是一种良好的纤维支架，可以用于构建组织工程瓣膜；内皮细胞种植在去细胞瓣叶 上构建组织工程心脏瓣膜是可行的。 关键词 组织工程；心脏瓣膜；人脐静脉内皮细胞 中图分类号 R654．2 Experimented study on creating acellular tissue engineering heart valve JIANG Hongliang，WU Xiuhua1,HUANG Lirong(Division of Thoracic and Cadiovascular Surgery，the First-Hospital of Yancheng，Jiang— SU 224001； Department of Pulmonary，the Sixth Hospital of Shanghai) ． Abstract Objective：To investigate the feasibility of creating tissue engineering heart valve from human umbilical vein en— dostelial cells(HUVECs)as an alternative cell source．MetlIods：Biomechanical characteristics offresh valves and acellularized Valves were tested ；HUVECs were cultured in vitro，and frequently seeded onto the acellularized scaffolds，and was their growth observed． Results：The acellularization procedure resulted in complete removal of the cells in valve leaflets while there was no significant differ— ence of biomechanical characteristics between them ．Th e matrix could be successfully seeded with expanded HUVECs in vitro，which formed a continuous monolayer on the surface．Conculsions：Acellularied porcine aortic valves can be applied as a scaffold to create tissue engineering heart valves．Generation of functional tissue engineering heart valves from HUVECs was feasible． Key words tissue engineering；heart valve；human umbilical vein endostelial cells 心脏瓣膜病最终需要进行瓣膜替换手术，机械 1．2 实验方法 瓣需终生抗凝，异种生物瓣耐久性差，同种瓣存在排 1．2．1 去细胞猪主动脉瓣叶的制备：(1)猪主动脉瓣 斥反应且供体来源困难【1]。组织工程为获得理想的心