布朗葡萄藻在不同培养基中的生长效应.pdfVIP

第26卷 第4期 生 态 科 学 26(4)：332-336 2007年8月 Ecological Science Aug． 2007 布朗葡萄藻在不同培养基中的生长效应 胡章喜，徐 宁，李爱芬，段舜山 暨南大学水生生物研究中心，广东广州510632 【摘要】 研究了布朗葡萄藻 (Botryococcus braunii) 764株和765株在3种培养基Chu10、Chul3x2和SE中的 生长效应。结果表明：(1)B．braunii 764在Chu10中的细胞密度、生物量、总脂含量和总烃含量均高于Chu13x2 和SE；B．braunii 765在Chu10中的细胞密度、生物量和总脂含量高于Chul3x2和SE，而总烃含量在Chul3x2中 较高。(2)B．braunff 764的总脂和总烃含量分别为19．4％、23．4％，显著高于B．bra“nii 765。 关键词：布朗葡萄藻；培养基；生长；总脂：总烃 中图分类号：Q949．226 2 文献标识码：A 文章编号：1008．8873(2007)04．332．05 Efleets of different culture medium on two Botryococcus braunii HU Zhang-xi，XU Ning，LI Ai-fen，DUAN Shun-shan’ Research Centre ofHydrobiology,Guangdong,Guangzhou，510632，Chh~a Abstract：Growth of Botryococcus braunii 764 and Botryococcus braunii 765 that were cultured in three media(Chul 0， Chul 3 x2 and SE)was studied and compared．．Cell density(OD6so)，biomass(dry weight)，total lipids，and total hydrocarbons were measured．The following results were reported：(1)total lipids and total hydrocarbons of B．braun~ 764 were 1 9．4％and 23．4％respectively,which were significantly higher than those of B．braunii 765；(2)cell density， biomass，total lipids and total hydrocarbons of B．braunii 764 that was cultured in medium Chul 0 were higher than those cultured in media Chul 3 x2 and SE，whereas only cell density，biomass and total lipids of B．braunii 765 cultured in medium Chul0 were higher than those cultured in Chul3x2 and SE，total hydrocarbons in Chul3x2 Was higher than those inChu10 andSE． Key words：Botryococcus braunii；culture medium；growth；total lipids；total hydrocarbons 收稿日期：2007．05．08，2007．07．20接受 基金项目：国家自然科学基金30370231)：广东省科技计划重点项目(2005：珠海市科技计划重点项目(13(220061045) 作者简介：胡章喜 (1982一)，男，博士生，主要从事藻类生物学和水环境生物学研究 }通讯作者，E-mail：tssduan@jnu．edu．cn 4期 胡章喜，等：布朗葡萄藻在不同培养基中的生长效应