抑制AtCLH1表达降低叶片衰老进程中叶绿素a与b的比值但不影响叶绿素降解速率.pdfVIP

596 JournalofPlantPhysiology andMolecularBiology 2007，33(6)：596-606 Repression of AtCLH1 Expression Results in a Decrease in the Ratio of Chlorophyll a／b but Doesn’t Affect the Rate of Chlorophyll Degradation during Leaf Senescence ZHOU Xiao，LIAO Yang，REN Guo—Dong，ZHANG Yan-Yan，CHEN Wen—Jun，KUAI Ben-Ke State Key Laboratory ofGenetic Engineering，Institute ofPlant Biology，School ofLife Sciences，Fudan University，Shanghai 200433，China Abstract：To explore the possible regulatory role of Tanaka 2006)，well known as the“PaO pathway’，named chloroph) llase(Chlase)in chlorophyll(Ch1)degradation after its pace—ma king enzyme，the pheophorbide a oxy— during leaf senescence，RNAi Arabidopsis rabidopsis genase(PaO)，whichis responsibleforopeningthering thaliana)plants were constructed to repress the expres— structure of pheophorbide a(Pru~inskfi et a1．2003)．The sions of AtCLH1 and／or AtCLH2．Transcript levels of first step of this pathway is considered to be catalyzed AtCLH1 and／or AtCLH2 were dramatically lowered and by chlorophyll—chlorophyllide(Chlide)hydrolase Chlase activity was correspondingly inhibited，but the (Chlase)(EC 3．1．1．14)．since the breakdownproducts of Chl degradation kinetics was not affected in the RNAi the Chl found in different plant species are mainly the plants．Results of further analysis indicated that the Chl structures without phytol(Amir—Shapira et a1．1987； a／b ratio decreased in AtCLH1 RNAi lines，in compari— Engel et a1．1991；Shioi et a1．1991)．Chlase also partici· son with the increasing Chl a／b ratio in the wide type pates in the Chl cycle which is coupled with Chl degra— during leaf senescence．In addition，an induced Chlase dation and responsible for the conversion of Chl(ide)b activity was consistently detected at the initial stage of to Chl(ide)a(Rudiger 2002)(Fig．1)