非诺贝特对高脂血症大鼠NO及血管内皮细胞粘附分子-1表达的影响.pdfVIP

· 1872· 南方医科大学学报(J South Med Univ) 2007；27(12) 非诺贝特对高脂血症大鼠NO及血管内皮细胞粘附分子．1表达的影响 吴 峻 ，孙 明 ，林锦潮 ，何兆初 ，区碧如 ，郭海森 ( 广州医学院第一附属医院心内科，广东 广州510120； 中南大学湘雅医院心内科，湖南 长沙410008) 摘要：目的 探讨血脂与炎性因子对内皮功能的损伤机制。方法实验包括正常对照组(基础饲料喂养)，高脂血症组(高脂 喂养)和高脂血症非诺贝特治疗组(高脂喂养)，其中非诺贝特治疗组在高脂喂养同时喂服非诺贝特40mg·(kg ·d )而高 脂血症组不予药物治疗。20周后检测3组的血脂、NO浓度及观察血管内皮血管细胞粘附分子．1(VCAM．1)的表达水 平和细胞粘附密度。结果与正常对照组比较，高脂血症组NO水平较低、血管内皮上白细胞粘附增多、VCAM．1表达强 度较强及范围较广。非诺贝特治疗组与高脂血症组比较，血NO水平提高、血管内皮VCAM．1表达水平和细胞粘附数 目均较低(少)。结论NO减少及炎症因素的介入参与了血管损害机制，非诺贝特能有效地阻止动脉硬化的发生，该作用 与NO水平提高、VCAM．1表达下调有关。 关键词：高脂血症：血管细胞粘附分子．1；N0；非诺贝特 中图分类号：R965；R972 文献标识码：A 文章编号：1673-4254(2007)l2．1872．03 Impact of fenofibrate on No and endothelial VCAM一1 expression in hyperlipidemic rats wu Jun ，SUN Min ，LIN Jin-chao ，HE Zhao·chu ，OU Bi·m ，GUO Hal·sen’ Department of Cardiology， First Affiliated Hospital of Guangzhou Medical College， Guangzhou 510120， Chma； 2Department of Cardiology，Xiangya Hospital of Central South University，Changsha 4 1 0008，China Abstract：Objective To investigate the mechanism of hyperlipidemia-and imflammation-induced functional impairment of the endothelium．Methods The experiment was conducted using 3 groups of rats fed for 20 weeks with standard chow(control group)，high-fat diet and high-fat diet with daily fenofibrate treatment(10 mg／kg，starting since the fifIll week)，respectively． After 4 and 20 weeks offeeding，respectively， serunl lipid level and NO concentration were measured in the rats，and the epithelial vascular eell adhesion molecule．1(VCAM．11 expression and eell adhesiveness to the aortic endothelium were observed．Results Compared with the control group，the rats with hyperlipidemia induced by long-term high-fat diet feeding showed lower N0 concentration and incre~ed leukocyte accumulation on the endothelial surface．exhibiting also stronger and more extensive endothelial expression of V