座壳孢代谢产物的抗菌活性与作用机制.pdfVIP

应用与环境生物学报 2007，13(6)：872～875 Cl^ JAppt Environ Biol=ISSN1006-687X 2007—12_25 座壳孢代谢产物的抗菌活性与作用机制 潘洁茹 张鸿声 邱君志 朱炎平 关雄 (福建农林大学生物农药与化学生物学教育部重点实验室 福州 350002) 摘 要 研究了座壳孢(Aschersonia Montagne)分离株JosO09代谢产物的抗菌活性和抗真菌作用机理，并比较了不同培 养方式对其代谢产物活性的影响．结果表明，JosO09液体培养的代谢产物有广谱抗细菌(最小抑制浓度从37．5到150 wg／mL)活性，发现座壳孢代谢产物具有抗植物病原真菌这一新的生物活性．经扫描电镜观察，该菌代谢产物可直接作 用于黄瓜枯萎病病原菌尖镰孢古巴专化型的细胞壁，导致细胞壁降解和坏死．此外，研究表明，液体培养比固体培养更 有利于抗菌活性物质的产生．图3表3参13 关键词 座壳孢；代谢产物；抗菌活性；扫描电镜；作用机制 CLC S‘476 Antimicrobial Activities and Mechanism of Metabolites from Aschersonia sp．JosO09 PAN Jieru，ZHANG Hongsheng，QIU Junzhi，ZHU YanpingGUAN Xiong一 (Key Laboratory ofBiopesticide and Chemical Biology，Fujian Agriculture and Forestry University， Ministry of Education，Fuzhou 350002，China) Abstract Antimicrobial activities and antifungal mechanism of the metabolites from Aschersonia sp．Jos009 were investigated， and comparative studies on the bioactivities of the metabolites under different cultivation conditions were conducted．The results showed that the metabolites produced by liquid cultivation displayed broad antibacterial spectrum and were active to some pathogenic fungi．，I1le minimal inhibitory concentrations(MICs)of the metabolites against sensitive bacteria ranged from 37．5 to 150 mL．，I1le antifungal mechanism of the metabolites against Fusarium oxysporum sp．cubense was further studied by scanning electron microscope (SEM)technique．Considerable morphological alterations，including degradation，blistering and necrosis in the hyphae exposed to the active metabolites．were found．The study showed that liquid cultivation was better than solid cultivation for the production of bioact