甘薯根腐病病原菌粗毒素对甘薯生长的影响.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 37(6)：604-609(2007) 甘薯根腐病病原茵粗毒素对甘薯生长的影响 柴一秋 ，陈利锋 ，王金生 ( 浙江省亚热带作物研究所，温州325005； 南京农业大学植物病理学系农业部病虫监测与治理重点开放实验室，南京21@5) 摘要：本文鉴定了茄病镰孢甘薯专化型[Fusarium solani(Mart)Sacc．f．sp．batatas McClure，简称FSB]引起甘薯胜利百 号根腐病以及诱导胜利百号显蕾开花，并研究了菌株V100—93-06粗毒素对胜利百号组培苗生长的影响。结果表明，菌株 V100—93-06对不同甘薯栽培品种呈现不同的致病性，其粗毒素能够诱导甘薯出现和病原菌寄生相似的部分症状。粗毒素 原液处理胜利百号薯苗2、3、5和9 d，病情指数分别为0．9,2．8、2．5和3．0，而l0倍稀释浓度的粗毒素处理2、3、5和9 d， 病情指数分别为0、1．5、2．8和2．5。l—l 000倍稀释浓度的粗毒素处理胜利百号组培苗3 h，就能够诱导出现黄叶，浓度越 高诱导出现黄叶数越多；对基芽和根的影响则表现为抑制基芽和根的生长，浓度越高基芽和根的数量越少；对根伸长生长 的影响表现为l倍稀释浓度粗毒素抑制根的伸长生长，l0～l ooo倍稀释浓度都能够促进根的伸长生长。 关键词：茄病镰孢甘薯专化型；甘薯；开花；粗毒素；组织培养 Effect of crude toxin of Fusarium solani f．sp．batatas on the growth of sweet potato CHAI Yi．qiu ，CHEN Li-feng ，WANG Jin-sheng ( Institute of Subtropical Crops of Zhejiang Province；Wenzhou 325005，China； Key Laboratory of Monitoring and Management of Plant Diseases and Insects，Ministry of Agriculture；Depart- ment of Plant Pathology，Nanjing Agricultural University，Nanjing 210095，China) Abstract：Fusarium solani(Mart)Sacc．f．sp．batatas McClure(abbreviation as FSB)was identified to cause root rot and induce flowering of sweet potato Shenglibaihao．The effect of crude toxin of strain V100-93— 06 of FSB on the growth of sweet potato tissue culture seedlings was studied．The results showed that the viru- lence of V100-93-06 to different varieties V撕ed．and its crude toxin resulted in similar symptoms as the fungal infection．The disease index of symptom of Shenglibaihao seedlings was 0．9，2．8，2．5 and 3．0 at 2 ，3 ， 5th and 9th d after treated with the crude toxin，and the disease index of symptom was 0，1．5，2．8 and 2．5 ， treated with 10 dilutions．~／hen the seedlings of tissue culture of Shenglibaihao treated with 1—1 000 dilutions of crude toxin，yellow leaves were induced only in 3 h，and the high concentration of crude toxin was used， the more yellow leaves was got；the crude toxin inhibited the growth