罗汉果皂苷Ⅲ的人肠内细菌生物转化.pdfVIP

北 京 大 学 学 报 ( 医 学 版 ) JOURNAL OF PEKING UNIVERSITY(HEALTH SCIENCES) Vo1．39 No．6 Dec．2007 ·657· · 论 著 · 罗汉果皂苷Ⅲ的人肠内细菌生物转化 杨秀伟 ，张建业 ，徐 嵬 (北京大学1．药学院天然药物学系，2．天然药物及仿生药物国家重点实验室，北京 100083) [摘 要]目的：探讨人肠内细菌对中药罗汉果中罗汉果皂苷Ⅲ结构的生物转化，确定转化产物的结构，为原形化 合物罗汉果皂苷Ⅲ在肠内吸收的评价提供科学依据。方法：采用人肠内细菌与罗汉果皂苷Ⅲ在厌氧条件、37℃共 温孵培养的方法对其结构进行生物转化，通过硅胶柱色谱技术分离、纯化生物转化产物，应用红外光谱(infra．red spectrometry，IR)、核磁共振波谱(nuclear magnetic resonance spectrometry，NMR)和质谱(mass spectroscopy，Ms)等技 术方法确定生物转化产物的结构。结果：罗汉果皂苷Ⅲ可由人肠内细菌依次脱去C-3葡萄糖基和C-24龙胆二糖基 转化产生罗汉果皂苷Ⅱ 。和罗汉果醇。结论：罗汉果皂苷Ⅲ可被人肠内细菌进行生物转化，产生次级苷罗汉果皂苷 Ⅱ ，及其苷元罗汉果醇。 [关键词]罗汉果；皂苷类；肠；细菌；生物转化 [中图分类号]R282．71 [文献标识码]A [文章编号]1671-167X(2007)06-0657-06 Biotransformation of mogrosideⅢ by human intestinal bacteria YANG Xiu-wei。 ZHANG Jian．ye。，XU Wei。 ， (1．Department of Natural Medicines，2．State Key Laboratory of Natural＆Biomimetic Drugs，Peking University School of Pharmaceutical Sciences，Bering 100083，China) ABSTRACT Objective：To investigate the biotransformation of mogroside 11I，one of the main chemical constituent in the fruits of Momordica grosvenori Swingle，by the crude enzymes of human intestinal bacte． ria．and determine the structure of biotransformation products and provide scientific basis for absorption e． valuation of primary mogroside 11I in human intestine．Methotis：The mogroside 111 was incubated with crude enzymes of human intestinal bacteria under the anaerobic environment and 37℃ condition to trans． form mogroside 11I．The biotransformation products were isolated and purified by silica gel column chro． matography，and structurally determined by infra-red(IR)and nuclear magnetic resonance(NMR) spectrometry and mass spectroscopy(MS)techniques．Results：Mogroside 111 was converted to mogro- side II Al and mogrol by successive deglycosylation at C-3 of the glucosyl group and C-24 of the gentiobio- syl group．Conclusion：Th