羊驼种群内亲缘关系的研究.pdfVIP

第16卷第6期 激 光 生 物 学 报 V01．16 N n 2007年 12 JJ ACTA LASER BIOLOGY SINICA De(-．2007 · 基础研究 · 羊驼种群内亲缘关系的研究 j 莉 ，赵英虎 ，董常生 (1．山西农业大学动物科技学院，山西太谷030801；2．中北大学信息商务学院，山西太原030051) 摘 要：应用RAPD技术对羊驼群体进行亲缘关系检测。在所采用的40条随机引物中筛选出l0条，优化了 RAPD的反应条件，确立了适合于羊驼基因组RAPD分析的最佳反应体系和反应程序。计算出个体问之间的带 纹相似系数及遗传距离指数，带纹相似系数位于0．3901～0．7851之间，平均为0．5374；彼此问的遗传距离最小 为0．2149，最大为0．6099，平均为0，4626，并绘制出个体间亲缘关系树状聚类图。结果显示羊驼种群内遗传基 础广泛，证明其个体间的血缘关系较远，这将有利于羊驼种群的正常生长、发育、繁殖和快速扩群。 关键词：羊驼；RAPD；亲缘关系 中图分类号：$813．3 文献标识码：A 文章编号：1007-7146(2007)06-0713-04 The Study on the Genetic Relationship in Population of Alpaca GAO Li ，ZHAO Ying—hu ，DONG Chang—sheng College ot Animal Science And Technology of Shanxi Agricultural University，Taigu 03080 1，Shanxi，China； 2．College of Information and Business of North University of China，Taiyuan 03005 1，Shanxi，China) Abstract：The genetic relationship was inspected on Alpaca individuals by RAPD．1 0 primers were screened from 40 Arbitrary primers，and reaction qualifications of RAPD were optimized．So the best reaction system and PCR program were established to adapt to Alpaca Genome RAPD．The correlation coefficient and the genetic distance were calculated based on it．hldices matrixes indicated that eorrelation coefficient was between 0．3901 and 0．7851，the average was 0． 5374，and the genetic distance was between 0．2149 and 0．6099 in 31 individuals，the average was 0．4626． Fhe poly— genetic tree of the genetic relationship in 3 1 individuals was drawn，it showed genetic base of Alpaca individuals was ex— tensi~re，and proved kinsheep a Alpacas is farther，which would be advantageous to the normal growth，development，re— production and rapid enlargement of Alpaeas． Key words：