胰蛋白酶在磁性核壳介孔分子筛上的固定化研究 - 食品科学.pdfVIP

266 2012, Vol.33, No.23 食品科学 ※生物工程 1 2 1 安 红 ，宋伟明 ，高树刚 (1.淮海工学院化学工程学院，江苏 连云港 222005 ；2.齐齐哈尔大学化学化工学院，黑龙江 齐齐哈尔 161006) 摘 要：采用吸附法研究胰蛋白酶在磁性核壳介孔分子筛(Fe O @MCM-41)上的固定化过程。考察吸附条件对胰蛋 3 4 白酶固定化性能的影响，并对固定化酶的酶学性能、载体结构等进行表征。结果表明：在固定化时间为4h 、胰蛋白 酶给酶量为60mg/g、pH8.2 的条件下，固定化酶活回收率可达55.9% 。表面能谱分析(EDS)结果表明，固定化酶中酶 含量( 以含氮量计)约为14%，与游离酶相比，固定化酶的耐温区间、pH值适应范围明显变宽，并具有一定的可重复 操作性，且固定后载体仍然保持良好的介孔结构。 胰蛋白酶；磁性核壳；介孔分子筛；固定化 Immobilization of Trypsin onto Magnetic Core-shell Mesoporous Molecular Sieve 1 2 1 AN Hong ，SONG Wei-ming ，GAO Shu-gang (1. College of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China； 2. College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China) Abstract ：A dipping method was used to immobilize trypsin onto magnetic core-shell mesoporous molecular sieve (Fe O @ 3 4 MCM-41). The effects of immobilization conditions on the recovery of trypsin activity were investigated and immobilized trypsin was analyzed for enzymatic properties and carrier structure. The recovery of trypsin activity reached 55.9% when immobilizing at pH 8.2 and an enzyme loading of 60 mg/g for 4 h. Energy dispersive X-ray spectroscopy (EDS) analysis showed that the immobilized trypsin contained 14% trypsin (calculated on the basis of total nitrogen content). Compared with free trypsin, it showed tolerance to temperature and pH in muc