正交试验优选马齿苋提取物制备工艺研究.pdfVIP

药学服务与研究 Pharm Care＆．Res 2008 Jun；8(3) 侯银环，等．正交试验优选马齿苋提取物制备工艺研究 · 论 著· 正交试验优选马齿苋提取物制备工艺研究 侯银环 ，辛海量。．- ，徐燕丰 ，岳小强 ，李 敏 ，路金才 ，凌昌全 (1．沈阳药科大学中药学院生药学教研室，沈阳110016；2．第二军医大学长海医院中医科，上海 200433；3．中国人民解放军第 546医院中医科，新疆马兰 841700；4．第二军医大学海医系军队卫生教研室，上海 200433) [摘 要] 目的：优选马齿苋提取物的制备工艺条件。方法：采用I 。(3 )正交试验设计，以药材中a一亚麻酸转移率、浸膏得 率和浸膏中 一亚麻酸的含量作为考察指标，采用 HPI C法测定 a亚麻酸含量，并对所得结果进行方差分析，综合3个指标结 果确定最佳工艺。结果：优选出的最佳工艺条件为将马齿苋提取液回收至无乙醇，加水至0．5 g／mI ，用lo 的Na()H溶液 调pH为7．5～8．0，5 000 r／min离心l min，沉淀加水至1 g／mI ，超声 10 min，5 000 r／min离心l min。结论 ：优选出的制备 工艺稳定可靠，简便可行。 [关键词] 马齿苋；a一亚麻酸；正交试验；工艺学，制药 [中图分类号] R 943 [文献标识码] A [文章编号] 1671-2838(2008)03 020l o3 Study on the optimization of preparation technology of extract of Portulaca oleracea L．with orthogonal test HOU Yin huan ，XIN Hal liang ’。，XU Yan feng。，YUE Xiao qiang ，1 1 Min ，I．U Jin—cai ，I．ING Chang—quan (1．Department of Pharmacognosy，School of Chinese Materia Medica，Shenyang Pharmaceutical University， Shenyang 110016，China； 2．Department of Fraditional Chinese Medicine，Changhai Hospital，Second Military Medical University，Shanghai200433， China；3．Department of Traditional Chinese Medicine，No．546 Hospital of PI A，Xinjiang Malan 841700，China；4．Department of Military Health，Faculty of Naval Medicine，Second Military Medical University，Shanghai 200433，China) [--ABSTRACT] Objective：To optimize the preparation technology of extract of Portula~，“o~gratea I… Methods：With extrac tion yield of d—linolenic acid，the yield of extract and the content of d linolenic acid in extract as criteria，whose content was de— termined by HPI C method，the preparation process conditions were optimized by I 9(3 )ortbogonal test．Results：The best preparation technology conditions were as follows；concentrating the extract under reduced pressure till there was no ethanol， adding water to 0．5 g／mL，adjusting pH to 7．5-8．0 with l0 NaOH solution，centrifuga