肌肉生成抑制因子多克隆抗体的制备与鉴定 - 华南农业大学学报.pdf

26 3 Vol.26, No.3 　　　　　　　　　　　 　　　　　　　　　　　 　2005 7 Journal of South China Agricultural University 　Jul.2005 马现永, 曹永长, 马静云, 毕英佐 (华南农业大学 动物科学学院, 广东 广州510642) :(myostatin, MSTN), , .Western- lot ELISA :20 μg/mL, 800 , P/N 2, , 1∶12 800, .400 , D450nm0.624, P/N 5.552. :;;Western- lot;ELISA :S856.65　　　　　　:A　　　　　:1001-411X (2005)03-0089-04 Preparation and identification ofpolyclonal antiserum against myostatin MA Xian-yong, CAO Yong-chang, MA Jing-yun, I Ying-zuo (College of Animal Science, South China Agric.Univ., Guangzhou 510642, China) Abstract:Oil-emulsion vaccines were prepared using purified MSTN protein, by which the rabbit was immu- nized four times to obtain MSTN-specifc antibody, which were then purified with ammonium sulfate salting-out procedure.Western-lot and ELISA assay results showed thatwhen the contentration of antigenwas 20 μg/mL and the dilution factor was 800, the mensured P/N was larger than 2, indicating that preparation of MSTN- specific antibody was successful and the titer of antibody was 1 ∶12 800.The specificity of the antibody was very high.When the dilution factor of antibody was 400, D450 nm was 0.624,P /N was 5.552. Keywords:myostatin;polyclonal antibody;Western-lot;ELISA 　　(MSTN) MSTN-Q、SOC-MSTN- , [ 1] [2] [5] .1997 ,McPherron . ,2 ～ 、、(leptin) [3] 3 .MSTN , [4] ;:(HRP) ogdanovich MSTN , ;( ,., 、) Sigma ;TM MSTN , . MSTN 1.2　 . 1.2.1　MSTN 纯 蛋白浓度的测定及其乳