osmyb 调控化感水稻酚酸类物质合成及其抑草作用 - 作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2015, 41(4): 531538 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2015.00531 OsMYB 调控化感水稻酚酸类物质合成及其抑草作用 沈荔花1,2 李碧凉1 任勇杰1 李程勋1 钟永嘉2 方长旬1,2 林文雄1,2,* 1 , 350002; 2 , 350002 : PI312777 (Oryza sativa L.) OsMYB (CT829537)(overexpression, OE)RNA (RNAi), (Echinochloa crusgalli , BYG) , PI312777 , CT829537-OEPI312777 , , ; CT829537-RNAiPI312777 , , , OsMYB (CT829537) : ; ; ; ; Phenolic Acid Synthesis of Allelopathic Rice Regulated by OsMYB and Its Weed Inhibition 1,2 1 1 1 2 1,2 SHEN Li-Hua , LI Bi-Liang , REN Yong-Jie , LI Cheng-Xun , ZHONG Yong-Jia , FANG Chang-Xun , and LIN Wen-Xiong1,2,* 1 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Institute of Agro-ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China Abstract: Regulation of gene expression is a vital process in the formation of rice allelopathy. In this study, allelopathic rice PI312777 (Oryza sativa L.) was modified by RNA interference (RNAi) and overexpression(OE) technologies to inhibit or en- hance gene expression of OsMYB (CT829537) in PI312777 respectively. The results showed that up-regulation of phenolic syn- thesis related genes was found in CT829537-OE transgenic PI312777 co-cultured with barnyardgrass (BYG), compared with that in wild type. However, the reverse was true in the CT829537-RNAi transgenic PI312777. Up-re