β-环糊精增敏三维荧光法鉴别真伪五指毛桃 - 现代食品科技.pdf

现代食品科技 Modern Food Science and Technology 2017, Vol.33, No.5 β-环糊精增敏三维荧光法鉴别真伪五指毛桃 陈立弟，文震，陈庆，何红霞，郑宗坤 (深圳大学化学与环境学院食品科学与工程系，广东深圳 518060) 摘要：为建立药食同源五指毛桃真伪品的三维荧光快速鉴别方法，研究 β-环糊精(β-CD)对五指毛桃提取液的荧光增强作用。五 指毛桃提取液在弱酸性条件下与β-CD 形成主客体超分子化合物，提高了约一个数量级的荧光强度，为五指毛桃的荧光测定创造了条 件。考察了五指毛桃与β-CD 质量比 m[(五指毛桃):m(β-CD)]、敏化体系pH 值、敏化时间和温度等因素对五指毛桃/β-CD 体系荧光光 谱的影响，正交实验确定了敏化最佳条件，即m(五指毛桃):m(β-CD)=1:10，敏化时间 1 h，pH 值为 4.25 ，敏化温度 60 ℃。在优化β-CD 敏化条件下，三维荧光等高线图谱特征、荧光峰位置、强度作为五指毛桃指纹识别的参考依据。建立了β-CD 敏化三维荧光光谱法鉴 定五指毛桃的方法，灵敏度高，重现性好，可应用于五指毛桃的真伪品的快速鉴别。 关键词：三维荧光；五指毛桃；β-环糊精；敏化 文章篇号：1673-9078(2017)5-293-297 DOI: 10.13982/j.mfst.1673-9078.2017.5.047 Identification of Radix Fici hirtae and Its Adulterants by β-cyclodextrin-sensitized Three-dimensional Fluorescence Spectroscopy CHEN Li-di, WEN Zhen, CHEN Qing, HE Hong-xia, ZHENG Zong-kun (Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China) Abstract: To establish an identification method for Radix Fici hirtae (RFH)-a homology of medicine and food—and its adulterants by using three-dimensional fluorescence spectroscopy, the effect of β-cyclodextrin (β-CD) on the enhancement of the fluorescence intensity of RFH extracts was studied. A new supramolecular host-guest compound was formed by β-CD and RFH extracts in a weak-acid medium, and the fluorescence intensity of RFH extracts was improved by an order of magnitude, creating conditions for the determination of the fluorescence of RFH. The influenc