苹果蠹蛾幼虫的形态与分子鉴定 - 苹果蠹蛾防控信息网.pdf

1 2 3 2 1 2 1* 2 2 2 2 1. 300071 2. 3. 1 Morphological and molecular identifi cation of larvae of Cydia pomonella (Lepidoptera, Tortricidae). Li Teng , 2 3 2 1 2 1* 2 2 2 Cai Bo , Song Wen , Xu Wei , Zhao Qing , Long Qionghua , Bu Wenjun , Liu Fuxiu , Han Yuchun , Li Weidong , Lin 2 Mingguang (1. College of Life Sciences, Nankai University, Tianjin 300071, China; 2. Hainan Entry-Exit Inspection and Quarantine Bureau; 3. Nanchong Jialing Agriculture and Animal Husbandry Bureau) Abstract The technique and method of DNA Barcoding was applied to make a molecular identifi cation of one larva, which was intercepted in an apple from Russian tourist by Sanya Entry-Exit Inspection and Quarantine Bureau. Partial sequence of mitochondrial DNA COI gene as DNA barcodes of the larva was amplifi ed and sequenced with primers LCO1490/HCO2198. By blasting with known data in GenBank and BOLDSYSTEMS v3, the result showed that the sequence of the intercepted specimen was 100%identified with one sequence of Cydia pomonella in GenBank and three sequences of C. pomonella in BOLDSYSTEMS v3. According to the sequence and the morphological characters,the larva was identifi ed as C. pomonella. Key words Cydia pomonella; larvae; morphological characters; DNA Barcoding; molecular identifi cation DNA 1 LCO1490/HCO2198DNA COI PCR GenBank BOLDSYSTEMS v3 GenBank 1100% BOLDSYSTEMS v3 3100%