朱奕潼，苗雅，何婷，钟远 - 欢迎访问中华老年多器官疾病杂志！.doc

·基础研究· Arc/Arg3.1在糖尿病脑病大鼠海马组织中的表达及其与认知功能改变的关系 朱奕潼，苗 雅，何 婷，钟 远* （上海交通大学附属第六人民医院老年病科，上海 200233） 【摘 要】目的 探讨糖尿病大鼠认知功能与海马Arc蛋白和β淀粉样蛋白（Aβ）表达的相关性，探讨突触可塑性在糖尿病脑病（DE）发病机制中的作用。方法 30只SPF级雄性8周龄大鼠随机分为对照组和糖尿病组，糖尿病组高脂饮食喂养4 周后腹腔注射链脲佐菌素建立2型糖尿病大鼠模型，观察不同阶段动物的体质量、空腹血糖（FBG）、空腹血清胰岛素（FSI），并计算胰岛素敏感指数（ISI）。实验末行水迷宫测试评估两组大鼠认知功能；酶联免疫吸附测定法、免疫组织化学法和蛋白质印迹法检测观察大鼠海马组织Aβ和Arc的表达。结果 糖尿病组大鼠在目标象限的探索时间较对照组缩短（P＜0.01），原平台穿越次数较对照组减少（P＜0.01）。糖尿病组Aβ表达较对照组升高（P＜0.01），Arc/Arg3.1表达降低（P＜0.01）。结论 糖尿病大鼠认知功能受损可能与Arc表达降低导致突触可塑性失衡加剧Aβ的沉积和神经元损伤有关。 β肽类；大鼠 【中图分类号】 R587.2 【文献标识码】 A 【DOI】 ? Expression of Arc/Arg3.1 in diabetic rat hippocampus and its relationship with cognitive function ZHU Yi-Tong, MIAO Ya, HE Ting, ZHONG Yuan* (Department of Geriatrics, Sixth People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China) 【Abstract】 Objective To analyze the correlation of cognitive function expression of Arc protein and amyloid-beta peptide (Aβ) in the hippocampus of diabetic rats, and to assess the role of synaptic plasticity in the possible pathogenesis of diabetic encephalopathy (DE). Methods Thirty male SPF SD rats (8 weeks old) were randomly assigned to diabetes mellitus group and control group. After 4 weeks of high fat diet feeding, the rats of diabetes mellitus group were injected intraperitoneally with streptozotocin at a dose of 30 mg/kg to establish diabetic model. Body weight, fasting blood glucose (FBG), and fasting serum insulin (FSI) were monitored at several stages, and insulin sensitivity index (ISI) was calculated. At the end of the experiment, water maze test was carried out to evaluate the cognition of all the rats. The expression of Arc and Aβ in the hippocampus were detected and observed by ELISA, immunohistochemistry and Western blotting. Results The diabetes mellitus group spend shorter time in the target quadrant (P＜0.01frequency of crossing the original platform site (P＜0.01than the control group. The expression level of Aβ was obviously higher, whi