二至天癸颗粒对IVF-ET患者卵巢颗粒细胞FSHRmRNA表达的作用.pdfVIP

提 要 目的：研究二至天癸颗粒对于体外受精-胚胎移植患者卵泡质量、卵巢反应性及 妊娠结局的影响及该药对于卵巢颗粒细胞中FSHRmRNA表达水平的影响。 方法: 将 65 例行 IVF-ET 治疗的肾阴虚证不孕症患者随机分为 2 组，治疗组 32 例（二至天癸颗粒联合Gn用药组）和对照组33例（安慰剂联合Gn用药组）。观察两 组患者卵巢颗粒细胞FSHRmRNA表达、肾虚症候积分变化、HCG日血清E2水平和单个 卵细胞 E2、Gn 的用量及治疗天数、获卵数、临床优质卵率、受精率、优质胚胎率、 临床妊娠率情况。 结果：治疗组患者肾虚症状得到明显改善，治疗组 Gn 用量及用药天数低于对照 组，单个卵雌激素水平高于对照组，优质卵率、优质胚胎率及临床妊娠率均高于对照 组，差异均有统计学意义 （P0.05）;治疗组卵巢颗粒细胞FSHRmRNA表达高于对照组， 差异均有统计学意义（P0.05）。 结论：在 IVF-ET 治疗周期，二至天癸颗粒可以改善患者肾阴虚症状，减少 Gn 用量，提高优质卵率、优质胚胎率，进而改善临床妊娠率。二至天癸颗粒可以提高卵 巢颗粒细胞FSHRmRNA表达，从而提高卵细胞质量，这可能是二至天癸颗粒改善IVF-ET 结局的机制之一。 关键词 二至天癸颗粒；IVF-ET；FSHRmRNA ；妊娠结局 Research of Erzhi Tiangui Granule to Effect FSHRmRNA on Ovarian Granulosa Cells in Vitro Fertilization-embryo Transfer Speciality: Gynecology of TCM，the Study of Female Sterility Author: Yangyang Zheng Tutor: Prof ．Fang Lian Abstract Objective: To explore the mechanism of Erzhi Tiangui Granule (ETG) in improving the quality of oocyte ，the ovarian response and Pregnancy Outcome by measuring the relationship of FSHRmRNA on ovarian granulosa cells in vitro fertilization-embryo transfer. Methods: In clinical study, 65 infertility patients of kidney yin deffciency undergoing IVF-ET cycles were randomly divided into 2 groups:the treatment group (32cases) was treated with ETG and Gn , the control group (33cases) was treated with placebo and Gn.the relationship of FSHRmRNA on ovarian granulosa cells ，the syndrome scores of TCM，administration time and number of Gn,thickness and type of endometrium, the number of fertilization, the fertilization rate,high quality embryo rate and pregnancy rate were evaluated. Statistical analysis was done by SPSS 17.0 for windows. Results: (1) Before treatment two groups we