高速逆流色谱法分离纯化红曲色素组分 - 食品科学.pdfVIP

192 2010, Vol. 31, No. 20 食品科学 ※工艺技术 1 1 2 2 2 1 , 2 , 郑允权 ，李泳宁 ，王阿万 ，陈芬玲 ，石贤爱 ，郭养浩 * (1.福州大学药物生物技术与工程研究所，福建 福州 350002 ； 2.福建省医疗器械与医药技术重点实验室，福建 福州 350002) 采用高速逆流色谱法(HSCCC)分离纯化红曲发酵产品中 6 种 Azaphilone 类色素组分。筛选弱极性分离溶剂 系统正己烷 - 醋酸乙酯 - 甲醇- 水，研究 6 种色素组分在不同溶剂体系中的分配系数，建立两步逆流萃取分离的技术 路线。经过 HPCCC 分离纯化和丙酮结晶操作，得到 6 种高纯度的 Azaphilone 类色素组分，纯度均大于 98.5% ，得 率达到 81.40%～84.78%，所得的6 种色素组分的摩尔吸光系数分别为 13313、13877、9380、9360、25621 、25849 L/(mol ·cm) 。本研究可提供一种新型的制备高纯度红曲Azaphilone 类色素组分的技术路线。 红曲色素；高速逆流色谱；分离纯化 Separation and Purification of Monascus Pigments by High-speed Counter-current Chromatography 1 1 2 2 2 1,2, ZHENG Yun-quan ，LI Yong-ning ，WANG A-wan ，CHEN Fen-ling ，SHI Xian-ai ，GUO Yang-hao * (1. Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350002, China； 2. Fujian Key Laboratory of Medical Instrumentation and Pharmaceutical Technology, Fuzhou 350002, China) Abstract ：Six azaphilone-type pigments were separated from Monascus-fermented solid medium and purified/fractionated by by high-speed counter-current chromatography (HSCCC). A solvent system with weak polarity involving four components n- hexane, ethyl acetate, methanol and water was selected and used for HSCCC. Based on a comparative analysis of partition coefficients of target compounds in different solvent systems, a two-step HSCCC routine was developed. After separation by HSCCC and crystallization with acetone, six azaphilone-type pigments with high purity were obtained. The purity of each pigment