大孔树脂分离纯化何首乌中二苯乙烯苷的.docVIP

LSA-21大孔吸附树脂富集何首乌中二苯乙烯的工艺研究 曹骋，*，王术玲，（广州中医药大学中药学院， 广州 510006） 摘要 目的：对何首乌二苯乙烯苷的吸附。方法：采用高效液相色谱法测定23,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷大孔树脂对二苯乙烯的吸附和解吸率。结果：结论：本研究结果可为实际生产提供理论依据。 关键词： 何首乌；二苯乙烯苷；大孔吸附树脂[中图分类号] R283.6 [文献标识码]A [文章编号]1005-9903（2011）10- Study on the Technology of Enrichment of Stilbene Glycoside in Polygonum multiflorum with LSA-21 Marcoporous Resin CAO Cheng , JIA Wei , WANGShu-ling, CHENFeng-lian, ZENGYuan-er （Traditional Chinese Pharmaceutical College of Guangzhou University of TCM, Guangzhou 510006) [Abstract] Objective: To study the conditions and parameters of purifying stilbene glycoside from Polygonum multiflorum Thunb.. Method: HPLC was applied to determine the content of 2,3,5,4’-tetrahydroxy-stilbene-2-O-?-D-glycoside, and calculate the adsorption rate and desorption rate. Result: The optimum process condition was as follows: 60% ethanol was used as eluting solvent; the adsorption velocity was 1.0 mL·min-1, and the solution concentration was 0.2 g·mL-1; elution velocity was 1.0mL/min. Conclusion: The desorption purification effect of LSA-21 marcoporous resin is good. So this method of purification is advisable. The results of this study can provide theoretical basis for practical production. [Key words] Polygonum multiflorum Thunb.; stilbene glycoside; LSA-21 marcoporous resin 何首乌为蓼科植物何首乌Polygonum Multiflorlum Thunb.的干燥块根，具有补肝、益肾、养血、祛风之功效，[1]。何首乌主要含有二苯乙烯苷类、蒽醌类、磷脂类、糖类等成分。二苯乙烯苷具有抗衰老、降低胆固醇、提高免疫功能、防治动脉硬化及保肝等作用。大孔吸附树脂吸附分离技术是一种纯化新工艺，可以达到分离、富集E-mail：caosir@126.com [通讯作者]*贾薇，讲师，博士，从事中药分析及质量标准研究，电话：E-mail：jiawei6636@ 中药有效部位或有效成分的目的。为了更好地发挥何首乌的药效，，为实际生产提供理论依据。 岛津LC-10AT高效液相色谱仪 SPD-10Av检测器C18色谱( 4.6mm×250mm，5μm)766-3型KETONG 远红外辐射干燥箱。何首乌（广州致信中药饮片有限公司，经鉴定蓼科植物何首乌Polygonum Multiflorlum Thunb.的干燥块根）2,3,5,4′ - 四羟基二苯乙烯-2-O-β-D-葡萄糖苷（中国药品生药制品检定所，批号110844-200606）；LSA-21（公司）乙腈Merck)，水为蒸馏水，其余试剂为分析纯加10倍体积50%乙醇，恒温水浴锅中加热回流提取2次，每次1h，合并提取液，滤过，滤液减压浓缩至无醇味，高速离心（3000r·min-1）取上清液，调节浓度至.2g/mL（相当于生药量）。流动相：乙腈-水（25 : 75）；检测波长320nm；柱温：室温；流速：1.0mL/minμl。 2.2.2对照品溶液的制备 取2，3，5，4′-四羟基二苯乙烯