parg抑制对小鼠结肠癌ct26细胞vegf-c蛋白表达调控的初步研究 .docVIP

PARG抑制对小鼠结肠癌CT26细胞VEGF-C蛋白表达调控的初步研究 吴伟强，王娅兰，颜佳欣，潘 娟（400016重庆，重庆医科大学病理教研室，分子医学与肿瘤研究中心） 目的 初步探讨多聚（腺苷二磷酸核糖）水解酶[poly-（ADP-ribose）glycohydrolase，PARG]抑制对小鼠结肠癌CT26细胞VEGF-C蛋白表达。方法 实验分为实验组（GLTN处理）与对照组（GLTN未处理），采用Western blot法检测组小鼠结肠癌CT26细胞的PARGPARP-1、NF-κB及VEGF-C的蛋白表达变化；RT-PCR分析实验组与对照组的VEGF-CmRNA水平变化；ELISA法检测组小鼠结肠癌CT26细胞上清VEGF-C蛋白表达变化。结果小鼠结肠癌CT26细胞表达的NF-κB的表达下降(P0.01)，PARP-1和VEGF-C蛋白较组(P0.05)。RT-PCR结果显示CT26细胞株±0.08）]较对照组[相对灰度值为（0.63±0.04）]显著下降(P0.01）。实验组小鼠结肠癌CT26细胞分泌的VEGF-C蛋白量95.86±5.43）pg/ml]较对照组1 99.32±0.75）pg/ml]明显降低（P0.05）。结论 在小鼠结肠癌CT26细胞中，抑制PARG可下调 PARP NF-κB的表达，从而使VEGF-C表达下降，这可能在抑制肿瘤淋巴管形成中起重要作用。 多聚（腺苷二磷酸核糖）水解酶多聚(腺苷二磷酸核糖)聚合酶-κB；血管内皮生长因子C肿瘤淋巴管形成 [中图分类号] R735.3+4 [文献标码] A Effect of PARG on Expression VEGF-C in Murine Colon Carcinoma CT26 Cells Wu Weiqiang，Wang Yalan，Yan Jiaxin，Pan Juan （Department of Pathology，Molecular Medicine and Cancer Research Center，Chongqing Medical University，Chongqing，400016，China）Abstract] Objective The objective of this article was to investigate the role of PARG inhibition in the regulation of VEGF-C expression in CT26 cells. Methods CT26 cells were treated with or without the PARG inhibitor Gallotannin (GLTN). The expressions of PARG, PARP-1, NF-κB, VEGF-C and β-Actin protein in CT26 cells were measured by Western blot analysis. The VEGF-C mRNA level was detected by reverse transcription polymerase chain reaction (RT-PCR).The level of VEGF-C secreted by murine colon carcinoma CT26 cells was measured by enzyme-linked immunosorbent assay (ELISA). Results The results of Western blot showed that the expression levels of PARG, PARP-1, NF-κB, and VEGF-C were reduced in GLTN-treated CT26 cells. The levels of VEGF-C mRNA in GLTN-treated CT26 were significantly weaker than that in GLTN-untreated cells (P 0.05). The VEGF-C levels in GLTN-treated CT26 cells culture supernatants were significantly weaker than that in GLTN-untreated CT26 cells culture supernatants (P 0.05). Conclusion Here, we provide evidence for the first time that PARG