丁型肝炎病毒抗原的表达纯化和抗原性分析.pdfVIP

丁型肝炎病毒抗原的表达纯化和抗原性分析 1 ,3 1 1 1 2 谢 　立 ,黄德庄 ,贺立香 ,罗朝霞 ,周育森 ( 1. 北京市卫生局肝炎研究所 ,北京 100054 ; . 2 军事医学科学院微生物流行病研究所 ,北京 10007 1 ;3 . 中国科学院研究生院 ,北京 100039) Expression , Purif ication and Antigenicity Analysis of the HD V Antigen XIE Li1 ,3 , HU AN G Dezhuang1 , H E Lixian g1 ,L U O Zhaoxia1 ,ZHOU Yusen2 ( 1. B eij i ng M unicip al H ep at it is I ns t it ute , B eij i ng 100054 , Chi na; 2 . I ns t i t ute of M icrobiology an d Ep i dem iology , Chi nese A ca demy of M i l it ary S cience , B eij i ng 10007 1 , Chi na; 3. Gra d uate S chool of t he Chi nese A ca demy of S ciences , B eij i ng 100039 , ) Chi na Abstract : A mp lified HDV f ragment by R TPCR wa s cloned into T vector and PQ E3 1 p la smid , w hich e st abli shed a p ro karyotic exp re ssio n vector in E col i M15 The reco mbinant p rot ein wa s p uri fied by NiN TA column affinit y chro mato grap hy , and identified by We st er n blot and EL ISA : HDV antigen wa s exp re ssed efficient ly an d had a well antigenicit y charact er . It can be u sed in HDV clinical diagno si s and epidemiolo gy survey . Key words : H ep at i t is D v i r us ( HDV) ; Gene cloning and exp re ssin g ; HDV antigen 关键词 :丁型肝炎病毒 ;基因克隆和表达 ;丁肝抗原 中图分类号 :R5 12 . 6 　　　文献标识码 :A 　　　文章编号 :10035 125 (2005) ( ) ( ) 　　丁型肝炎病毒 H ep at i t is D v i r us , HDV 是一 RN A 酶抑制剂 RN a sin 、Taq DN A 聚合酶 、随机 ( ) 种缺陷负链 RN A 病毒 , 其表面被 乙肝病毒抗原