大骨节病关节软骨细胞凋亡及其相关调控因子的表达 作者：王世捷；郭 .docVIP

大骨节病关节软骨细胞凋亡及其相关调控因子的表达 作者：王世捷；郭雄；左弘；张银刚；许鹏；平智广；张增铁；耿冬　　(西安交通大学环境与疾病基因教育部重点实验室/环境与地方病研究室，陕西??西安??710061) 摘要：目的??探讨大骨节病患者关节软骨细胞凋亡及相关调控因子Bcl-2、Bax、Fas及iNos表达分布的特点。方法??收集15例大骨节病儿童和15 例正常对照儿童关节软骨。采用脱氧核糖核酸末端转移酶介导的脱氧核糖核酸缺口标记(TUNEL)技术和Bcl-2、Bax、Fas及iNos蛋白免疫组化抗生物素蛋白-生物素-碱性磷酸酶(B-SA)法染色，观察大骨节病儿童和正常对照儿童关节软骨的凋亡细胞和Bcl-2、Bax、Fas及iNos蛋白阳性表达细胞的密度与分布。结果??(1)大骨节病儿童关节软骨中层凋亡阳性细胞数[(33.60±2.71)%]较正常关节软骨[(1.33±0.41)%]显著增多(t=11.59，P0.01)；(2)大骨节病儿童关节软骨表层和中层Bcl-2、Bax、Fas及iNos的表达显著高于正常关节软骨中的表达(t=11.75~18.65，P0.01)；大骨节病儿童关节软骨各层Bcl-2、Bax、Fas及iNos表达阳性率有显著性差异(F =73.49~114.42，P0.01)，以表层最多[分别为(41.93±12.26)%、(45.60±15.78)%、(53.60±16.49)%和(45.47±14.02)%]，其次为中层[分别为(14.93±3.50)%、(13.87±4.32)%、(23.27±4.83)%、(21.67±6.82)%]。结论??大骨节病患者关节软骨细胞凋亡及相关调控因子Bcl-2、Bax、Fas、iNos表达较正常人显著增多。 关键词：大骨节病；软骨，关节；细胞凋亡；Bcl-2；Bax；Fas；iNos 中图分类号：R684.101; R394.2??文献标识码：A??文章编号：1000-2588(2005)06-0643-04 Chondrocyte apoptosis and the expression of Bcl-2, Bax, Fas and iNos in articular cartilage in Kashin-Beck diseaseWANG Shi-jie； GUO Xiong； ZUO Hong； ZHANG Yin-gang； XU Peng； PING Zhi-guang； ZHANG Zeng-tie； GENG DongKey Laboratory of Environment and Genes Related Diseases, Institute of Environment and Endemic Diseases of State??Ministry of Education, Xi’an Jiaotong University, Xi’an 710061, ChinaAbstract: Objective??To investigate the characteristics of chondrocyte apoptosis and distribution of Bcl-2, Bax, Fas and iNos expressions in articular cartilage in Kashin-Beck disease (KBD). Methods??Samples of articular cartilage were collected from 15 healthy children and 15 children with KBD diagnosed according to the Pathological Criteria of KBD Diagnosis in China. Chondrocyte apoptosis was detected by TUNEL method, and the articular chondrocytes positive for Bcl-2, Bax, Fas and iNos were stained by B-SA immunohistochemistry. Results??The percentage of apoptotic chondrocytes positively stained by TUNEL in the middle layer of articular cartilage was significantly higher in KBD children than in the control group (33.60%±2.71% vs 1