穿琥宁对原发性血小板增多症患者巨核细胞体外扩增的影响.pdfVIP

内科急危重症杂志 2008年 第14卷 第3期 151 穿琥宁对原发性血小板增多症患者巨核细胞体外 扩增的影响 宜昌市第一人民医院 高开波 张红胜 冯春 韩莉 黄志 刘旭，宜昌 443000 摘要 目的：研究穿琥宁注射液对原发性血小板增多症患者巨核细胞体外扩增的影响。方法：采集12例原发 性血小板增多症患者骨髓及血小板计数正常的缺铁性贫血患者骨髓，分离出CD34 细胞，接种于IMEM悬浮培养 体系中，试验组及对照组加入 TPO、II 一1B、IL一3、IL一6、FW3L和不同浓度剂量的穿琥宁注射液，分别在3、7、10、14d 收集培养扩增的细胞，通过流式细胞术检测巨核细胞表型及数量，血浆块法检测 巨核细胞集落形成单位(CFU— MK)数。结果：试验组巨核细胞扩增倍数及 cFU—MK的形成能力随着穿琥宁剂量的递增逐渐减弱，呈明显量效关 系，并明显弱于未加穿琥宁组及对照组；对照组未呈现量效关系。巨核细胞在7～10d扩增速度最快，14d时减慢。 巨核祖细胞(CD34 CD41 细胞)在7d时最多，在10～14d时下降。结论：穿琥宁对原发性血小板增多症患者骨髓 中巨核细胞增殖有抑制作用。 关键词 穿琥宁 原发性血小板增多症 巨核细胞 扩增 中图分类号 R558 文献标识码 A Effect of Chuan‘Huning on Amplification of Megakaryocytes in Patients With Essential Thrombocytosis in Vitro GAO Kaibo，ZHANG Hongsheng，FENG Chun，et a1．Yichang First Peoples Hospital，Yichang 443000，China Abstract Objective．．To study the effect of Chuan—Huning on amplificatin of megakaryocytes in patients with essentia1 thrombocytosis(ET)in vitro．Methods：CD34 fraction were collected from bone marrow cells of 12 es— sential thrombocytosis patients and 1 2 iron deficiency anemic patients with normal blood platelets count and cultured in IMEM media supplemented with TPO，II 一1B，IL一3，II 一6 and Fit一3L．Different concentrations of purified Chuan— Huning solution were added into liquid culture while the same volume of saline served as contro1．Suspension cells were harvested after 3，7，1 0 and 1 4 days incubation respectively and examined for megakaryocyte marker by flow cytometry and megakaryocytic colony—forming unit(CFU—MK)． Results：The number of megakaryocytes and the CFU—MK in ET group were greatly inhibited by Chuan-Huning compared to control group．This effect was most significant following 1 4 days of culture and was dose dependent．The megakaryocyti