生防菌诱导水稻抗病性相关酶活性的变化.pdfVIP

仲恺农业技术学院学报，21(2)：o6—08，2008 Jounm／ Zhongkad Un／vers／ty ofAgr／cu／ture and Techno／ogy 文章编号：1006—0774(2008)02一OOO6—03 生防菌诱导水稻抗病性相关酶活性的变化 游春平，肖爱萍，刘开启，彭13聪 (仲恺农业技术学院植物保护系。广东广州510225) 摘要：将生防菌bi0—2和稻瘟病菌 (Magnaporthe gr／sea)接种于正处于分蘖期的水稻植株上，测定其与植物抗病 有重要相关的苯丙氨酸解氨酶 (Phenlyalanine ammonia lyase PAL)、过氧化物酶 (Peroxidase，POD)和多酚氧化酶 (Polyphenoase，PPO)活性的变化．结果显示，接种处理后，POD活性明显高于对照，在接种后第5 d，稻瘟病菌、 稻瘟病菌+生防菌bio一2处理组的POD活性达到最大值，但到接种后第8 d开始略低于对照；稻瘟病菌+生防菌 bio-2、生防菌bio-2处理组，其叶片内的PAL活性仅在第1—2 d内高于对照，其后均低于对照；在稻瘟病菌+ 生防菌bio一2、生防菌bio一2处理组，其PPO活性除在第2 d高于对照外，其余均低于对照． 关键词：短小芽孢杆菌 (Bac／／／uzpum／／uz)；稻瘟病；诱导抗性；酶 中图分类号：$436；$476 文献标识码：A Dynamic of resistance-related enzymes in rice induced by antagonistic bacterium YOU Chun·ping，XIAO Ai—ping，LIU Kai—qi，PENG Ri—tong (Department of Plant Protection，Zhongkai University of Agriculture and Technology，Guangzhou 510225，China) Abstract：Rice plants on tiHefing stage were inoculated with antagonistic bacterium bio-2(S)or／and Magmrporthe gri~ea(C)for analyzing the activities of the indicators：phenlyalanine ammonia lyase (PAL)，peroxidase(POD)and polyphenoase(PPO)，which weI1e closely related with plants resist— alice to diseases．The results showed that the activity of POD Was higher than that of the control，and the peaks ofPOD activity treated with(C)and(C+S)appeared on the 5也d，but lower than that ofthe control on the 8也d The activity of PAL and PPO Was lower than that of the control except for treatments ． with C+S and S．whose activity at 1“or 2 d Was higher than that of the contro1． Key words：Bac／／／u．s pumilus；rice blast；inducing resistance；enzyme 稻瘟病是由稻瘟病菌 (Magnaporthe gri~ea)引起的水稻真菌性病害，是水稻的主要病害之一，流行年 份一般可使水稻减产10％一20％，严重者达40％一50％以上。并降低水稻品质u J．目前防治稻瘟病比较 成熟的措施主要是选择抗稻瘟病较强的品种、运用水稻品种多样性控制稻瘟病的发生和流行，以及采用化