高铜对雏鸭肾脏细胞周期和凋亡影响的研究.pdfVIP

畜牧兽医学报 2008．39(7)：980—984 Affa terinaria et Zootechnica Sinica 高铜对雏鸭肾脏细胞周期和凋亡影响的研究 崔 伟，彭 西，赵 丽，杨 帆，崔恒敏 (四川农业大学动物医学院，雅安 625014) 摘 要：360只1 f_l龄天府肉鸭随机分为6组，分别喂以对照13粮(Cu 8 mg／kg)和高铜13粮(Cu 100 mg／kg，高铜 I组；Cu 200 mg／kg，高铜Ⅱ组；cu 400 mg／kg，高铜Ⅲ组；Cu 600 mg／kg，高铜Ⅳ组；Cu 800 mg／kg，高铜V组)6周， 以流式细胞术研究高铜对雏鸭肾脏细胞周期和凋亡的影响。结果显示，肾脏细胞的调亡比例随13粮铜水平的升高 而升高，当日粮铜水平达到400 mg／kg以上时，肾脏细胞凋亡比例与对照组比较差异极显著(PO．01)。日粮铜水 平达到400 mg／kg以上时，肾脏细胞的增殖指数(PI)极显著低于对照组(P％0．01)。结果表明日粮铜水平达到400 mg／kg以 时会引起肾脏受损，肾脏细胞的增殖分化严重受阻，肾脏细胞凋亡大量增加。 关键词：高铜；肾脏；流式细胞术；细胞凋亡；细胞周期；雏鸭 中图分类号：$856．9；$852．3 文献标识码：A 文章编号：0366—6964(2008)07—0980—05 Effect of Dietary High Copper on the Cell Cycle and Apoptosis of Kidney in Ducklings CUI Wei，PENG Xi，ZHAO Li，YANG Fan，CUI Heng～min (College of Veterinary Medicine，Sichuan Agricultural University，Yaan 625014，China) Abstract：The experiment was conducted with the objective of examining the effect of dietary high copper on the cell cycle and apoptosis of kidney in ducklings by the methods of flow cytometry (FCM)．360 one—day—old Tianfu meat ducklings were randomly divided into six groups and fed on diets as follows：Control(Cu 8 mg／kg)and high copper(Cu 100 mg／kg，high copper group l；Cu 200 mg／kg，high copper groupⅡ；Cu 400 mg／kg，high copper groupⅢ；Cu 600 mg／kg，high cop— per groupⅣ；Cu 800 mg／kg，high copper group V)for six weeks．Kidney samples were collected， then the cell cycle and apoptosis were tested by FCM．The results showed that the percentage of renal cell apoptosis was increased in high copper groupsⅢ，Ⅳ and V when compared with that of control group．The G。／GI phase of cell cycle of renal cell was lower，and S，G2+M phase was higher in high copper groups I andⅡthan that in control group．The G0／G1 phase of cell cycle of renal cell was much higher，and S，G2+M phase was