201B中药合剂对大鼠抗肾小球基底膜肾炎病变影响.docVIP

201B中药合剂对大鼠抗肾小球基底膜肾炎病变影响【摘要】 目的 观察201B中药合剂防治大鼠抗肾小球基底膜(GBM)肾炎的疗效。方法 建立大鼠抗GBM肾炎模型。实验分3组：肾炎+201B处理组、肾炎对照组及正常对照组。大鼠一次性尾静脉注射抗GBM抗血清后即刻给予201B中药合剂 (0．46g/(kg#8226;d)，灌胃，至第21天。对照组则给予等量的生理盐水。定期于第4天、第14天和第21天，检测大鼠尿蛋白、血清肌酐和尿素水平，观察肾组织病理学改变。结果 肾炎对照组鼠注射抗血清后于第4天即出现异常蛋白尿，血清肌酐和尿素亦持续上升；肾小球内可见细胞数增加和新月体形成，肾小管内大量蛋白管型，GBM呈不规则增厚。而上述病变经201B中药合剂处理后均明显好转。结论 201B中药合剂能够明显改善大鼠抗GBM肾炎的肾功能。? 【关键词】 201B； 抗GBM肾炎；大鼠?? Effects of 201B on anti-glomerular basement membrane nephritis in rats SUN Wei, TANG Ren-xian, ZHOU feng, et al.Department of Immunology and Microbiology, Xuzhou Medical College, Xuzhou 221004, China? 【Abstract】 Objective To investigate the effects of 201B on anti-glomerular basement membrane(GBM) nephritis in rats. Methods Anti-GBM nephritis model was elaborated by rabbit anti-rat GBM antibody in jection in SD rats in this study. The rats were divided into three groups: 201B treated group(0.46gokg-1od-1,ig), untreated nephritis control group and normal control group. 201B was administered daily after injection of anti-GBM IgG. All the rats were observed urinary protein at the 4th day, the 14th day and the 21st day. At the same time, the kidney specimens were collected. Results Rats injected with rabbit anti-GBM Ab developed heavy proteinuria by 4th days, and serum creatinine and serum urea appeared which kept on the rising. Glmerular hypercellularity, crescents, and protein casts were observed in nephriticrats. By electron microscopy, the thickening of GBM and loss of foot processes were seen. But, all pathological changes obviously turned for the better in 201B treated group. Conclusion 201B could inhibit the progression of rats anti-GBM nephritis.? 【Key words】 201B; Anti-GBM nephritis; Rats ? 人类新月体性肾炎是一种自身免疫性疾病，发病机制复杂。临床上多数患者起病较急，病程迅速，预后较差[1]。由于机体免疫异常，造成了肾炎病变的迅速发展，最终导致患者肾功能衰竭[2]。目前临床上治疗该疾病时，一方面积极对症处理，另一方面使用一些免疫抑制剂(如环孢素A、FK506等)控制疾病的发展，从而缓解病情[3-4]。但经长期大量资料证实，免疫抑制剂用于治疗肾小球肾炎等疾病时，疗效虽满意，可毒副作用较大，且价格昂贵。寻找切实有效的治疗方法急不可待。? 201B中药合剂的主要成分是益母草、黄芪和白茅根。此合剂经临床初试30多例肾小球肾炎患者，结果发现既