小叶丁香中橄榄苦苷的纯化工艺研究-食品科学.PDF

56 2010, Vol. 31, No. 08 食品科学 ※工艺技术 邓瑞雪，刘 普，袁 华，赵尤曼，段文录，尹卫平* (河南科技大学化工与制药学院，河南洛阳 471003) ：目的：研究新药材小叶丁香抗肝纤维化有效成分橄榄苦苷的富集纯化工艺。方法：以HPLC 方法检测， 以对照品橄榄苦苷含量为跟踪指标，比较6 种不同厂家、不同型号的大孔吸附树脂对小叶丁香中橄榄苦苷的吸附与 解析条件，优化最佳纯化工艺。结果：D-101 树脂大孔吸附树脂对小叶丁香中的橄榄苦苷具有较好的吸附分离效 果，经富集后橄榄苦苷的含量可以达到30% 以上。结论：D-101 型大孔树脂对小叶丁香中的裂橄榄苦苷具有较好 的吸附能力，用40% 乙醇溶液解吸附快速有效。树脂的重复利用度好，方法简单方便，适于工业化生产。 ：小叶丁香；橄榄苦苷；大孔吸附树脂；H P L C Macroporous Resin Adsorption for Purification of Crude Oleuropein Extracted from Syringa pubescens Turcz. DENG Rui-xue ，LIU Pu ，YUAN Hua ，ZHAO You-man ，DUAN Wen-lu ，YIN Wei-ping* (School of Chemical Engineering and Pharmaceutics, Henan University of Science and Technology, Luoyang 471003, China) Abstract：Macroporous resin adsorption was used to obtain high-purity oleuropein from Syringa pubescens Turcz., an effective antihepatic fibrosis component. Six common macroporous absorption resins were compared for differences in adsorption and desorption performance. D-101 resin was found to be the best choice for oleuropein purification. To achieve an optimal oleuropein purification, the adsorption and desorption characteristics of D-101 resin were investigated. Results showed that the purity of purified crude Syringa pubescens Turcz. oleuropein using D-101 resin under optimized adsorption-desorption conditions was more than 30%. Forty percent aqueous ethanol solution was a fast and effective solvent for oleuropein desorption. D-101 resin exhibited good reusability. This method is easy and convenient in operation and has promising potential for industrial application. Key words ：Syringa pubesceus Turcz. ；oleuropein ；macroporous absorption resin ；HPLC 中图分类号：R284.2 文献标识码：A 文章编号：1002-6630(2010)08-0056-04 橄榄苦苷是一种重要的裂环环烯醚萜苷类化合物， (Oleaceae) 丁香属(Syringa ) 植物，产