乳腺癌血管生成拟态及MVD、MMP-2表达及预后关系.doc

乳腺癌血管生成拟态及MVD、MMP-2表达及预后关系【摘要】目的：研究乳腺癌中是否存在血管生成拟态（VM），及其与基质金属蛋白酶（MMP-2）表达及预后的关系。 方法：选取乳腺癌石蜡标本180例，进行PAS和CD34双重染色，观察是否存在VM；然后对存在VM者和对照组进行MMP-2染色、乳腺癌微血管密度（MVD）计数以及随访，并比较他们之间的相互关系。 结果：9例标本中存在VM。VM组的生存率和MVD值明显低于无血管生成VM组。而VM组的MMP-2值明显高于无VM组。 结论：乳腺癌中存在VM，可能是高度恶性生物学行为的基础之一。 【关键词】血管生成拟态；乳腺癌；MVD；MMP-2 文章编号：1009-5519（2007）18-2693-03 中图分类号：R36 文献标识码：A The relationship of breast cancer vasculogenic mimicry with microvessel density MMP-2 expression and prognosis ZHANG Xu，CHEN Xuan-shi，LIU Jun (Department of Pathology，Luzhou Medical College，Sichuan 646000，China) 【Abstract】Objective: To study whether there was vasculogenic mimicry(VM) in breast cancer and its relationship with microvessel density(MVD)，MMP-2 expression and prognosis. Methods：180 paraffin specimens of breast cancer were chosen to perform double staining of PAS and CD34 and VM were observed. And then specimens existing VM and the controls were performed MMP-2 staining and MVD counting， and the patients from whom the specimens were obtained were followed up. The relationship of breast cancer VM with MVD， MMP-2 expression and prognosis were compared. Results：9 specimens were found existing VM. The survival rate and MVD of the VM group were obviously lower than those of the non-VM group. And the MMP-2 expression of VM group was significantly higher than that of non-VM group. Conclusion：VM might be one of the foundations of its highly m alignant biological behavior. 【Key words】Vasculogenic mimicry；Breast cancer；MVD；MMP-2 乳腺癌是女性生殖系统发病率居首位的恶性肿瘤，关于其血供途径的研究对乳腺癌诊治有很重要的作用。目前的研究主要集中在肿瘤诱导血管生成（即Angiogenesis）方面，而对于新近发现的在具有高度侵袭性和转移性肿瘤中除血管生成外还可以形成模拟的血管通路，称之为血管生成拟态（vasculogenic mimicry，VM)，与乳腺癌的关系研究较少。我们拟通过CD34和PAS双重染色技术观察在乳腺癌中是否存在VM，并进一步研究VM与乳腺癌微血管密度（microvessel density，MVD）、基质金属蛋白酶-2（MMP-2）表达及预后之间的关系。 1 材料与方法 1.1 标本与试剂：样本来源于2000年1月～2002年1月四川泸州医学院附属医院及附属中医院乳腺癌根治术病例。存档石蜡标本中，挑选出随访资料完整的浸润性导管癌标本共180例，另取乳腺良性肿瘤瘤旁正常乳腺组织20例。每例标本均进行切片复习。CD34 和MMP-2为鼠抗人单克隆抗体，Santa Cruz公司生产。免疫组化采用EnVision二步显色法，DAKO公司生产。PAS染色液为本室配制