怡生汤对小鼠肠道菌群功能影响探究.doc

怡生汤对小鼠肠道菌群功能影响探究【摘要】 目的：研究怡生汤对小鼠肠道菌群的影响方法：小鼠随机分为4组：正常组、怡生汤低、中、高剂量组，口服给予14d, 观察怡生汤对小鼠肠道内双歧杆菌、乳杆菌、肠杆菌和肠球菌、产气荚膜梭菌、拟杆菌等6种菌群数目的变化结果：在连续给予怡生汤14d后,与给药前相比较，怡生汤中、高剂量组小鼠肠道内双歧杆菌、乳酸杆菌和拟杆菌的数目明显增多(p＜0.05或p＜0.01)，肠球菌、肠杆菌和气荚膜梭菌的的数目明显减少 (p＜0.05) 结论：怡生汤促进小鼠肠道中益生菌的生长增殖, 同时抑制有害致病菌的生长，对肠道菌群有一定的调节作用。 【关键词】 怡生汤；肠道菌群；双歧杆菌 The study of the regulatory effect of yi sheng tang on intestinal flora in mice Chen JianwenZhou ChanghuaLichengGuojianZengshenTiantianChen Tingting （School ofPharmaceutical Science，Sun Yet-sen Universty, Guangdong Guangzhou,510080） 【Abstract】Purpose: This study was to investigate the effects of yi sheng tang on the intestinal micro flora in mice. Methods: Mice were randomized into 4 groups:control group, the low 、 medium and high dose group.The yi sheng tang groups were orally administered with yi sheng tang at different doses and the control group was orally administrated with distilled water instead. Then the numbers of Bifidobacterium, Lactobacillus, Enterococcus, Enterobacter, C.perfringens etc were counted respectively. Results: This study suggests that in the medium and high dose group, the administration of yi sheng tang for 14 consecutive days can greatly stimulate the proliferation of probiotic bacteria such as Bifidobacterium, Lactobacillus , Bacteroid and down-regulate pernicious bacteria such as Enterococcus, Enterobacter andC.perfringens. Conclusion:The yi sheng tang can make an improvement to the alteration of intestinal flora. 【Key words】yi sheng tang; intestinal flora; bifidobacterium 本研究拟通过比较灌胃前后正常小鼠体内肠道菌群变化，研究怡生汤对小鼠肠道菌群的影响，以期为怡生汤的应用为提供实验依据。 1材料与方法 1.1材料 怡生汤：由党参，茯苓，白术，猴头菇，低聚木糖组成 (煮成水煎液 0.5g/mL生药量)，4℃保存，给药前复温至25-30℃。实验动物:SPF级雄性BALB/c小鼠48只，20±2.0g，由广州中医药大学实验动物中心提供（合格证号：SCXK(粤)2008-0020）。培养基: PTYG、MRS、Ec、EMB、NN、GAM。仪器：双人单面超净工作台，苏州净化；YQX-II型厌氧培养箱，上海予腾；恒温水浴锅，北京优莱博；高压灭菌锅，北京安捷来。 1.2方法 1.2.1 实验动物分组与给药 健康雄性BALB/c小鼠48只随机分为4组：正常对照组、低剂量组、中剂量组、高剂量组。按照怡生汤的成人日服剂量0.15g/ kg的10倍剂量设定中剂量组( 1.5g/ kg) , 上、下各设一个剂量组: 低剂量组( 0.75g/kg) 、高剂