白蛋白对幼兔胎粪吸入综合征保护作用及机制探究.docVIP

白蛋白对幼兔胎粪吸入综合征保护作用及机制探究【摘要】目的：探讨白蛋白对胎粪吸入综合征的保护作用及机制。方法：将28只幼兔分为模型组(n=12)、白蛋白组(n=12)和正常对照组(n=4)，建立幼兔胎粪吸入模型；在胎粪吸入后8、16、24小时计数支气管肺泡灌洗液(BALF)白细胞(WBC)、中性粒细胞(PMN)，并采用放射免疫法测定肺组织匀浆液及BALF中IL-8含量，在显微镜下观察肺损伤的程度。结果：同对照组相比，模型组BALF中PMN和WBC数升高，肺组织匀浆液和BALF中IL-8含量明显升高，差异有极显著性(P0．01)，且肺组织病理改变明显。同对照组相比，白蛋白组上述指标呈现类似改变，而同模型组相应时间点相比，白蛋白组上述指标降低，差异有极显著性(P0.01)，肺损伤程度明显减轻。结论：幼兔胎粪吸入后，肺部产生明显的炎症反应，IL-8在胎粪吸入综合征的发生发展中占有重要的作用；白蛋白可通过减少IL-8的分泌，保护胎粪吸入所致的肺损伤。 【关键词】胎粪吸入综合征；白细胞介素－8；白蛋白；幼兔 文章编号：1009-5519（2007）19-2846-03 中图分类号：R33 文献标识码：A Protective effect of albumin and its mechanism on meconium aspiration syndrome in immature rabbits HUANG Mao1，DONG Wen-bin1，XU Ya-ji2，FENG Xiao-ping3 (1.Department of Newborn Medicine．The Affiliated Hospital of Luzhou Medical College，Sichuan 646000，China；2.Teaching and Research Section of Physiology,Luzhou Medical College，Sichuan 646000，China；3.Department of Newborn Medicine，The People’s Hospital of Leshan，Sichuan 614000，China) 【Abstract】Objective：To investigate the protective effect of albumin and its mechanism on meconium aspiration syndrome in immature rabbits．Methods：Twenty-eight immature rabbits were randomly divided into three groups：model group (12 rabbits)，albumin group (12 rabbits) and control group(4 rabbits)．The animal model was established by endotracheal intubation imbuing 20% meconium (0.6 ml/kg)．At 8.16.24 hours after instillation，the number of white blood cells(WBC)，polymorphonuclear neutrophils(PMN)in bronchoalveolar lavage fluid (BALF)was calculated respectively：the level of IL-8 in lung tissue and BALF was measured by radioimmunoassay；and the pathological changes of lung tissues were observed under microscope. Results：Compared with control group．WBC，PMN，IL-8 were significantly increased in model group(P0.01)．The pathological changes of lung tissues were obvious．These variables of albumin group were similar with control group at every detecting point；compared with model group however，these variables were significantly decreased along wi