结缔组织生长因子在肾血管性高血压大鼠心肌中表达及意义.docVIP

结缔组织生长因子在肾血管性高血压大鼠心肌中表达及意义[摘要] 目的：研究结缔组织生长因子(CTGF)的表达与肾血管性高血压(RVH)大鼠心肌肥厚的关系，探讨CTGF在RVH大鼠心肌肥厚发生、发展过程中的作用。方法：用两肾一夹方法构建RVH大鼠模型。构建成功的RVH大鼠随机分为RC1及RC2组，同时设置对照组（SC组）。以心肌胶原含量、左心室重量(LVW)、左心室重重与体重比值(LVW/BW)作为心肌肥厚的指标，应用免疫组化法检测CTGF蛋白的表达。结果：①RVH大鼠术后第8周出现心肌肥厚，第12周进一步加重。②RC1和RC2 组CTGF蛋白表达显著增加（较假手术组）；且RC2较RC1组大鼠CTGF蛋白表达增加更显著。结论：肾血管性高血压大鼠CTGF表达增多，提示CTGF参与了肾血管性高血压大鼠心肌肥厚的发生。 [关键词] 高血压；肾血管性；心室肥厚；结缔组织生长因子 [中图分类号] R544.1+4[文献标识码]A[文章编号]1673-7210（2008）07（b）-013-03 The relation between connective tissue growth factor and ventricular hypertrophy in the rats with renovascular hypertension SUN Hong-lei1，ZHANG Jing-qun2，MA Ye-xin2 (1.Central Hospital of Jiaozuo Coal Group Co.Ltd.，Jiaozuo 454150，China；2. The Affiliated Tongji Hospital，Tongji College of Huazhong University of Science and Technology，Wuhan 430030，China) [Abstract] Objective:To study the relation between connective tissue growth factor and ventricular hypertrophy in the rats with renovascular hypertension. Methods: Some two-kidney one-clip (2K1C) renovascular hypertension（RVH）rats were established. Then they were randomly divided into two groups:RC1 and RC2. In addition，sham-operated rats were designed as controls(SC). The left ventricular weight(LVW) and the ratio of left ventricle weight to body weight(LVW/BW) were measured when the rats were killed at the endpoint of the study. The CTGF protein in the left ventricular myocardium were assayed by immunohisto- chemical methods. Results: ①Significant ventricular hypertrophy appeared at the 8th week and became more serious at the 12th week after surgery.②The expression of CTGF protein in the left ventricular myocardium increased significantly in RC1 rats and RC2 rats(compared with the sham rats)，and the expression of CTGF protein in RC2 rats increased more significantly than in RC1 rats. Conclusion:Increased expression of CTGF protein in left ventricular myocardium of rats with renovascular hypertension indicate that CTGF may play important roles in the development