维药老鼠瓜中主要硫苷提取及抗风湿作用.docVIP

维药老鼠瓜中主要硫苷提取及抗风湿作用[摘要] 目的：对维药老鼠瓜中的硫苷进行提取分离，研究它对类风湿性关节炎大鼠的影响。方法：提取分离老鼠瓜中的硫苷， Freunds 完全佐剂诱导法建立风湿性关节炎大鼠模型，设立正常组、模型组、雷公藤组、硫苷低、中、高剂量组(25，50，100 mg/kg) ，测量各组大鼠继发侧足厚度，进行疼痛评分和多发性关节炎指数评分。结果：本文制备了高纯度的硫苷结晶，经光谱鉴定确证为白花菜苷（glucocapparin）。与模型组相比，硫苷组大鼠佐剂性关节炎继发病变显著受抑制(P＜0.05)。结论：硫苷对大鼠类风湿性关节炎有良好的治疗作用。 [关键词] 老鼠瓜；硫苷；白花菜；大鼠；佐剂性关节炎 [中图分类号] R593.22 [文献标识码]B [文章编号]1673-7210（2008）02（c）-034-02 Extraction and anti-rheumatism effect of glucosinolates in the Capparis spinosa L. JIANG Li-Juan1，MEI Li-WanABDRYM 1*，HajiAkberAISA2 (1.School of Pharmaceutical Sciences， Xinjiang Medical University， Urumqi 830054，China; 2.Xinjiang Technical Institute of Physics and Chemistry， Chinese Academy of Sciences， Urumqi 830011， China) [Abstract] Objective:Extraction and separation of the glucosinolates from the Capparis spinosa L， discuss the effect of glucosinolates on adjuvant arthritis rats.Methods:The dry seed power was extracted with boiling MeOH， a glucocapparin was obtained by the recrystallization. Rats were randomly divided into the normal group and the model group， the small， medium， large dosages of glucosinolates group (25， 50，100 mg/kg)，Except for the rats in the normal group， animals were modeled to adjuvant arthritis with freunds complete adjuvant. The arthritis index (AI)， the pain response andthe parameter of paws were recorded. Results:UV1，1H-NNR， 13C-NNR was set up to identify the structure. At last we got the crystallization of glucocapparin. Compared with the model group， glucosinolates was shown to exert a dramatic inhibitory effect on secondary reaction of Freund’s adjuvant arthritis of rats.Conclusion:Glucosinolates has a remarkable treatment effect on AA rats. [Key words] Capparis spinosa L.; Glucosinolates; Glucocapparin; Rat;Adjuvant arthritis 老鼠瓜(Capparis spinosa L.)为白花菜科山柑属植物,又名槌果藤、刺山柑、野西瓜、钩瓜，在我国主要分布在新疆、甘肃和西藏[1]。其味辛苦，性温，具有祛风、散寒、除湿、止痛、调经、消肿的作用，维吾尔医用老鼠瓜治疗痛风和风湿性关节炎[2]。硫苷（glucosinolates，LG）是老鼠瓜主要的化学成分[3，4]，本文从老鼠瓜种子中提取分离硫苷类成分，考察了该组分对大鼠佐剂性关节炎(adjuvant arthritis，AA