脑心康注射液急性毒性实验探究.docVIP

脑心康注射液急性毒性实验探究[摘要] 目的:观察脑心康注射液是否会对小鼠产生急性毒性反应。方法:记录小鼠尾静脉注射药物的最大浓度和最大体积,24 h内给药3次,连续观察14 d。结果:小鼠行动正常,未出现躁动、发声;未发生举尾、振颤、痉挛、运动失调、姿态异常等神经系统反应;无眼球突出、流涎、流泪、排尿、下泻、竖毛、皮肤变色等;眼、鼻、口腔无异常分泌物;饮水及食欲正常;体重变化正常;无死亡。结论:24 h内给予脑心康注射液0.841 8 g(生药量)/20 g(小鼠体重)对小鼠无明显毒性,该用药量是临床用药剂量的84～140倍。 [关键词] 脑心康注射液;急性毒性实验;实验研究 [中图分类号]R965 [文献标识码] A[文章编号] 1673-7210(2009)08(a)-063-02 Experimental study on acute toxicity of Naoxinkang Injection ZHANG Ying1, CHENG Yanwen2, QI Defa2, CHEN Yuquan2, QU Fujun2 (1.Department of Pharmacy, Heilongjiang Construction Hospital, Harbin150040, China; 2.Department of Pharmacology, the Second Hospital of Harbin Medical University, Harbin150086, China) [Abstract] Objective: To observe whether Naoxinkang Injection had acute toxicity to mice. Methods: The largest drug concentration and the maximum size of tail vein injection were recorded, the injection frequency was 3 times per 24 hours, observed continuously for 14 days. Results: The behavior of mice was normal, and wasn’t restless, vocal; there was no reaction of the nervous system such as tail, tremor, convulsions, ataxia, abnormal posture; and no exophthalmos, salivation, tears, urine, collapse, erect hair, skin discoloration, and so on; there was no abnormal secretions of eyes, nose and mouth; the appetite and the water-drinking were normal; body weight was normal; no deaths accured. Conclusion: There was no significant toxicity in mice when given the Naoxinkang Injection within 24 hours at the dose of 0.841 8 g(dosage of crude drug)/20 g (mouse’s body weight). The dosage in the experiment was 84-140 times as that in the clinical. [Key words] Naoxinkang Injection; Acute toxicity; Experimental study 脑卒中是临床常见病,是危害中老年人健康的疾病之一。近年来,中医药研究者在前人积累的丰富经验的基础上,运用现代科学技术对治疗脑卒中的中药复方进行研究,取得了很大的进展。脑心康是由人参、黄芪、灵芝、水蛭组成的纯中药制剂,具有益气固本、气充血运、活血利脉、补脑益智的功效,能促进学习记忆的恢复、改善临床症状、改善血液流变性,是治疗脑卒中的有效中药制剂[1]。临床常用的剂型有胶囊剂、片剂、注射用粉针剂等,口服制剂存在吸收缓慢、生物利用度低等缺点,注射用粉针剂不溶性微粒较多。因而,将其开发成注射液将更有制剂优势和疗效优势。 1 实验材料 1.1 实验药品 脑心康注射液:规格为5 ml/支