Angiopietin-2在胎儿生长受限患者胎盘组织中的表达及意义.pdfVIP

· 34· 中国优生与遗传杂志2008年第16卷第2期 Angiopietin一2在胎儿生长受限患者胎盘组织中的表达及意义 李 帆，冯 玲 (华中科技大学同济医学院附属同济医院妇产科，武汉 430030) 摘 要：目的 检测胎儿生长受限 (FGR)患者胎盘组织中血管生成素一2(ansiopoietin一2，Ang一2)的表达水平， 并探讨其与FGR的关系。方法 采用免疫组织化学方法 (SP法)和逆转录聚合酶链反应 (RT—PCR)检测FGR组和对照 组的胎盘组织中Ang一2表达的定位和表达量的差异。结果 1．S—P：(1)Ang一2在胎盘的绒毛滋养细胞、血管内皮细 胞及血管周围细胞均有表达。(2)FGR组Ang一2的平均光密度较正常对照组显著下降 (P0．05)。2．RT—PCR：FGR 组Ang一2 mRNA的表达量下降，于正常对照组相比有明显意义 (P0．05)。结论 妊娠晚期Ang一2水平显著下降可能 是FGR发病机制中的一个重要因素。 关键词：胎儿生长受限 ；血管生成素一2；SP法；逆转录聚合酶链反应 中图分类号：R714．58 文献标识码：A 文章编号：1006—9534(2o08)02—0034—03 The expression of angiopoietin一2 and its significance in placenta of pregnant women with FGR．L／Fan，FENG Lin (Dept．ofObstet and Gynecol，TongCi Hospital，Tongii Medical College Hua—Zhong University ofScience and Technology，Wuhan， 430030) Abstract：Objective：To detect the expression of angiopoietin一2(Ang一2)in placentas，and to evaluate the role of Ang一2 in FGR．Methods：Immtmohistochemistry(SP)was used to investigate the distribution and location of ansiopoietin一2 and the semi— quantitative method of reverse transcription polymerase chain reaction(RT—PCR)was employed to investigate the mRNA expression levels ofansiopoietin一2 in placenta tissues of normal pregnant women and women with FGR．Results：1．S—P：(1)Ang一2 Wilt8 located in trophoblast，endothelial cells and paravascular cells of placentas．(2)The mean density of ansiopoietin一2 in group of FGR Was significantlylowerthanthatin normal group(P0．05)．2．RT—PCR：Comparedwiththe normal group，themRNA expression levels of angiopoietin一2 w鹊reduced(P0．05)．Conclusion：The expression of Ang一2 was greatly reduced in late pregnancy，It maybe play an important role in the pathogenic causes of preeclampsia． Key words：Fetal growth restriction(FGR)；Angiopoietin一2(Ang一2)；SP；RT—PCR 胎儿生长受限(fetal growth restriction，FGR)是围生期主 龄正常体重的第10个百分位数。B型超声检查双顶径、头 要并发症之一，其围生儿死亡率为正常儿的4—6倍，不仅影 围、胸围、腹围、股骨长度等指