学校代码10062学号200505100306本科毕业论文.doc

学校代码：1 0 0 6 2 学 号：200505100306 本科毕业论文 UNDERGRADUATE DISSERTATION 论文题目：醋酸铅对传代培养大鼠骨髓间充质干细胞生长的影响 TITLE Effect of lead acetate on the growth of rat bone marrow mesenchymal stem cells under subculture 院 系 公共卫生学院 专 业 预防医学 年 级 2005级 学生姓名 于维莉 指导教师 杨昆 副教授 2010年6月 醋酸铅对传代培养大鼠骨髓间充质干细胞生长的影响 【摘要】目的 了解醋酸铅对传代培养大鼠骨髓间充质干细胞(Marrow mesenchymal stem cells，MSCSPF级龄SD(Spraque-Dawley)雄性大鼠40只，体重60g-70g，将随为组白对组铅剂组(15mg/kg·bw)剂组(30mg/kg·bw)剂组(60mg/kg·bw)灌胃染毒检测SPSS16.0统计学软件包对实验结果进行方差分析和直线相关分析。结果 染铅组铅浓对组，有统计学义(P0.05)染铅组细获1d时，OD值差别不(P0.05)；从3d起细进数长期6d起增殖速度明显减进剂铅组对组(P0.05)，细胞从4d起增殖加快，进数长期对组7d，细显减慢进MSC损伤作用较大。醋酸铅MSC生长速度减慢，生长能力减弱，骨髓MSC对于铅的毒性作用极为敏感，铅暴露应早期干预。 【关键词】醋酸铅；骨髓间充质干细胞；传代培养 Effect of lead acetate on the growth of rat bone marrow mesenchymal stem cells under subculture 【Abstract】Objective To ffect of lead acetate on the growth of rat bone marrow mesenchymal stem cells (Marrow mesenchymal stem cells, MSC) under subculture, and to give a comprehensive understanding of the injury of lead to the hematopoietic system, in order to provide the basis for further clarifying its mechanism and taking prevention and control measures from a new perspective. Methods In this study, 40 healthy male SD (Spraque-Dawley) rats, SPF level, 4 weeks old and weighting 60g-70g, were divided into four groups by body weight: control group, low dose of lead acetate (15mg/kg?bw), middle-dose group (30mg/kg?bw) and high-dose group (60mg/kg?bw), orally exposed. After subculture in complete medium, the cellular morphologies of the second generation in each group were observed, and the growth curves of each group were detected for the third generation, Then the variance and linear correlation on the experimental results were analysed by statistical package SPSS16.0. Results Blood lead concentrations of the-three lead-ex