内质网分子伴侣GRP94-GRP78及XBP1在病理性瘢痕中表达探析.doc

内质网分子伴侣GRP94\GRP78及XBP1在病理性瘢痕中表达探析[摘要]目的:检测内质网应激反应的关键分子葡萄糖调节蛋白94(GRP94)、葡萄糖调节蛋白78(GRP78)和X-盒结合蛋白1(XBP1)mRNA在病理性瘢痕的表达,探讨其基因表达及内质网应激反应与病理性瘢痕形成及转归的关系。方法:用RT-PCR法检测GRP94、GRP78和XBP1 mRNA在:①瘢痕疙瘩(13例)、增生性瘢痕(17例)和正常皮肤(15例)组织以及体外培养的瘢痕疙瘩(5例)、增生性瘢痕(5例)和正常皮肤(6例)成纤维细胞中的表达;②体外培养瘢痕疙瘩和增生性瘢痕成纤维细胞(各5例)中经0.1mg/ml氢化可的松作用前后的表达。结果:①GRP94、GRP78和XBP1 mRNA在瘢痕疙瘩、增生性瘢痕和正常皮肤组织及其成纤维细胞中的表达均无显著性差异(P0.05);②0.1mg/ml氢化可的松作用后,GRP94 mRNA在瘢痕疙瘩和增生性瘢痕来源的成纤维细胞中的表达量均显著下降,具有统计学意义(P0.05)。结论:内质网分子伴侣GRP94、GRP78和XBP1在瘢痕疙瘩和增生性瘢痕组织及其成纤维细胞中基因转录与正常皮肤组织及其成纤维细胞中基因转录水平一致; GRP94 mRNA的表达量显著下降可能是糖皮质激素治疗病理性瘢痕的机制之一。 [关键词]葡萄糖调节蛋白94;葡萄糖调节蛋白78;X-盒结合蛋白1;瘢痕 [中图分类号]Q591.2[文献标识码]A[文章编号]1008-6455(2010)03-0358-03 A preliminary study on the expression of endoplasmic reticulum chaperone GRP94、GRP78 and XBP1 in pathologic scar NIE Fang-fei,QIN Ze-lian,CHEN Dong-ming,ZHAO Xia (Department of Plastic Surgery,Peking University Third Hospital,Beijing 100191,China) Abstract: ObjectiveTo detect the expression of glucose regulated protein 94(GRP94),glucose regulated protein 78(GRP78) and X-box binding protein 1(XBP1), which are key molecules in endoplasmic reticulum stress,in pathologic scar,and to explore the association between their expression and pathologic scar. Methods The samples consisted of three kinds of tissues,which were keloid,hypertrophic scar and normal skin. Fibroblasts were derived from the samples and cultured in vitro.Hydrocortisone in 0.1mg/ml concerntration was administrated to the fibroblasts derived from keloid and hypertrophic scar. RT-PCR was used to assess the mRNA expression levels of the aimed genes in tissue samples and fibroblasts.Results No significant differences were detected on GRP94,GRP78 or XBP1 mRNA levels in tissues or fibroblasts among the three groups: keloid,hypertrophic scar and normal skin.GRP94 mRNA levels were lessened remarkably in each fibroblast sample after being treated with hydrocortisone,while the changes of GRP78 and XBP1 mRNA levels were irregular.ConclusionsThere are no