flow injectionsequential injection analysis systems potential use as tools for rapid liver diseases biomarker studyinjectionsequential流动注射分析系统潜在使用工具快速肝脏疾病生物标志物研究.pdfVIP

Hindawi Publishing Corporation International Journal of Hepatology Volume 2012, Article ID 281807, 8 pages doi:10.1155/2012/281807 Review Article Flow Injection/Sequential Injection Analysis Systems: Potential Use as Tools for Rapid Liver Diseases Biomarker Study Supaporn Kradtap Hartwell Chemistry Department, Xavier University, 3800 Victory Parkway, Cincinnati, OH 45207, USA Correspondence should be addressed to Supaporn Kradtap Hartwell, kradtas@ Received 15 October 2011; Accepted 17 December 2011 Academic Editor: Neil Guha Copyright © 2012 Supaporn Kradtap Hartwell. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Flow injection/sequential injection analysis (FIA/SIA) systems are suitable for carrying out automatic wet chemical/biochemical reactions with reduced volume and time consumption. Various parts of the system such as pump, valve, and reactor may be built or adapted from available materials. Therefore the systems can be at lower cost as compared to other instrumentation-based analysis systems. Their applications for determination of biomarkers for liver diseases have been demonstrated in various formats of operation but only a few and limited types of biomarkers have been used as model analytes. This paper summarizes these applications for different types of reactions as a guide for using flow-based systems in more biomarker and/or multibiomarker studies. 1. Introduction all other body systems, many substances/chemicals in the body may respond to the malfunctioning